Abstract: The paper reports two new species of the genus Ophiostoma H. & P. Sydow collected by the author in recent years. Chinese descriptions, latin diagnoses and figures for each species are given. They are growing on the wood of Pinus rnassoniana. Wood staining of P. massoniana is associated with the Ceratocystis pinicola G. H. Zhao sp. nov.. Perithecia are superficial and with the bases immersed in the agar plate. The black base is globose and 126. 9～183. 3 μm in diameter. The superficies of the ornamented with net-like brown hyphae, 5～6 μm in diameter. The neck of the perithecia is black, straight or slightly curved. There are various ornaments on the neck. Its dimensions are 84.4～169.2μm in length, 26.9～40.4μm in diameter at the base, 16.8～33.7 μm at the tip. Without ostiolars hyphae, the asci are difficult to find. Ascospore are unicellular, hyaline, oblong, without gelatinous sheath and its dimensions are (3.4～3.9)μm×(1.9～2.6) μm. Wood staining of P. massoniana is relation to the C. lignicola G. H Zhao sp. nov.. The perithecia are superficial or immersed in PDA medium. The black base is globose and 87～169.2μm in diameter. The surface of the perithecia is smooth. The neck of the perithecia is straight or slightly curved, crude, 287～352 μm in length, 23.6～54. 6 μm in diameter at the base, 20～20.6μm at the tip. Ostiolar hyphae absent. The asci are difficult to find. Ascospores are unicellular, hyaline, oblong, without gelatinous sheath, and its dimensions are (3.7～4.4)μm×(2.4～3.0) μm.