Abstract: The influence of rhizosphere medicine in different components and application methods on the survival rate, height, DBH, relative turgidity of bark(RT), morbidity, contagious index and tree capacitance of Populus hopeiensis were studied. The results showed that the rhizosphere medicine could increase survival rate, height, DBH, relative turgidity of bark and tree capacitance and decrease morbidity and contagious index dramatically. Two kinds of application methods were adopted: one was called the soil-mixed method and another called the root-dipping method. The soil-mixed method was more effective than the rootdipping method. The medicine was composed of fungus, IBA, compound fertilizer and aquasorb. The effects of four components of medicine on the growth and health of trees were different. Finally, we made sure the more effective proportion of four components of the medicine that was composed of 74. 997% fungus, 10. 000% aquasorb, 14. 999% compound fertilizer and 0. 004G IBAo