Abstract: Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China, United States, England, South Africa and Chile by using RAPD analysis method. 135 scorable DNA fragments were amplified with twelve random primers by PCR (Polymerase Chain Reaction) technique and the polymorphic score was 96.3%. The genetic dendrogram based on RAPD analysis showed that the all S. sapinea isolates could be clustered into three groups. The isolate CWS41 from Chile was separated genetically as a first group away from other isolates, and isolates F2 and J2 from China were as the second group. The third RAPD group accommodated other rest isolates (including B morphotype CWS43 from United States).