南京林业大学学报(自然科学版) ›› 2006, Vol. 30 ›› Issue (03): 105-108.doi: 10.3969/j.jssn.1000-2006.2006.03.024

• 研究论文 • 上一篇    下一篇

福建山樱花不定芽诱导和植株再生规模化繁殖试验

吕月良1,2,陈璋2,施季森1*,黄宇翔3,刘金燕3,谢建丽3   

  1. 1. 国家林业局、江苏省林木遗传基因工程重点实验室南京林业大学, 江苏 南京 210037; 2. 福建省林业厅, 福建 福州 350003; 3. 福建省农业科学院作物研究所, 福建 福州 350013
  • 出版日期:2016-06-18 发布日期:2016-06-18

Adventitious Bud Inducing and Plant Regeneration of Cerasus campanulata Maxim. in Large Scale

Lv Yue-liang1,2, CHEN Zhang2, SHI Ji sen1*, HUANG Yu-xiang3, LIU Jin-yan3, XIE Jian-li3   

  1. 1. The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu ProvinceNaniing Forestry University, Nanjing 210037, China; 2. Forestry Administration of Fujian Province, Fuzhou 350003, China; 3. Crop Institute of Fujian Academy of Agriculture, Fuzhou 350013, China
  • Online:2016-06-18 Published:2016-06-18

摘要: <正>研究了切根和截顶对湿地松苗木质量的影响,结果表明:(1)不同的切根处理对湿地松苗高和地径的生长有显著抑制作用,几种处理中,以9月份切根对苗高的抑制最小,以3次切根的处理对地径生长的抑制作用最小,但切根处理对湿地松苗木的高径比影响较小;(2)几种切根处理中,只有1次切根处理下地上部分的干重大干对照,2次切根处理根系干重最小。全苗千重以1次处理时最大,其次为对照;(3)不同的截顶处理下,湿地松苗高和地径生长都比对照低,单次截顶的苗高均比2坎截顶的高,地径生长以9月中旬处理的较大;(4)几种截顶处理中。根干重和侧须根干重都比对照低,其中8月中旬截顶处理下苗木生长最慢,单次截顶处理的侧主根干重均大于对照,全苗干重都明显小于对照。

Abstract: The explants in 4 em length collected from spring shoots of 3-year-old selected tree of Cerasus carnpanulata were inoculated on MS culture medium with 6-BA and IBA for adventitious buds mutiplication. The results showed that explants were prevented effectively from being brown and dying when the medium of 1/4MS were used. Explants grew fast and no vitrification when the medium plused with 1.0 mg/L 6-BA and 0.01 mg/L IBA. The medium of MS with 1.0 mg/L 6-BA and 0.3 mg/L GA3 was the best for multiplication and multiplication rate could reach to 600% during one culture cycle. Plantlets were to be transferred on the 1/2MS medium with 1.0 mg/L NAA and 0.2 mg/L IBA for rooting, when the explants were reached in about 4 cm. The root system could be developed well during 25 days and to be come a new plant. The rooting rate could reach to 92.1% after 35 days. The survival rate was above 94% when the plantlets were to be moved to nursery. The medium in 340//00 pearlite and 67%peat soil was good for rooting. This micropropagation technology could be applied in mass propagation.

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