Abstract: The changes of seasons and the fraction of vegetative storage proteins (VSP) in the branchs of Ginkgo biloba L. were measured and analyzed by microscope and SDS-PAGE. The results indicated that VSP accumulated in fall and kept high level throughout the winter, until the next spring the bud broke, VSPs were almost mobilized completely. With new shoots growing, VSPs resumed to accumulate in late summer and early fall. The fraction of VSP in the branchs of G. biloba L. could be divided into three different types: high molecular weight, medium molecular weight and low molecular weight. The proteins with high molecular weight 45kD changed greatly during the course of the shoots sprouting, of which the proteins 45kD could be detected clearly before it sprouted and disappeared gradually after the leaves spreading. The proteins 45kD were probably the main types of vegetative storage proteins of G. biloba L.