<正>分别用槐果碱、苦参碱、槐胺碱、苦豆碱、野靛碱、N-甲基野靛碱、莱曼碱和总碱配制成生物碱浓度为l×10-4g/mL的PDA培养基，并在培养基上接种灰葡萄孢菌后再接种松材线虫，观察、记录松材线虫繁殖的情况。结果表明，苦豆碱、野靛碱和总碱对线虫的抑制繁殖作用都较强，其中，苦豆碱能完全抑制线虫的繁殖，且能引起线虫的死亡。对测定的生物碱的分子结构及对松材线虫的活性的分析进一步验证了关于双稠哌啶类生物碱对松材线虫的活性与其分子结构中的官能团对之间关系的假说。

The main applications of GPS in forest inventory and surveying are the positioning of sample plot centre and the boundary positioning of sub-compartment. This paper studied the founding of the forest district GPS control network, calculated the conversion parameters of coordinate, then utilized the portable GPS to position the sampling plot centre and subcompartment boundary in forest land. The results indicated that five plots were enough for calculating the conversion parameters of coordinate; and the largest RMS error of single plot positioned by portable GPS was 3.86 m. The average boundary displacement and absolute displacement of sub-compartment’s center were 3.23 m, 3.72 m respectively and the area ac curacies of three sub compartments were higher than 98%. Such a method can meet the requirement of the GPS application in forest inventory and surveying. Moreover, this method is easy and practical and can improve the efficiency of forest inventory and surveying.