Abstract: Tissue culture technique of 36 plus-tree selected from Chinese fir progeny tests was reported in this paper. Among the 36 plus-tree 5 superior clones were systematically studied and their technique system of tissue culture was established completely. The techniques were as follows: Initial culture media was 1/2MS+6-BA(0.3～0.8mg/L)+Sucrose (3%), clump shoots induction culture media was 1/2MS～6-BA(0.5～0.7mg/L)+IBA(0.1～0.3rag/L)+Sucrose (3%), rooting culture was 1/2 improvement MS+IBA (0.5～1.0rag/L) or 1/2 improvemen, MS+ABT1#(1.0～1.5mg/L)+Sucrose (1.50%), and transplantation media was yellow-center soil 90%+fine sand 10%. Their rooting rate was up to 90%, and transplantation survival rate was up to 96%.