Abstract: Research of biological characters of 12 wood ectomycorrhizal fungi were carried out, in order to confirm the optimum growing temperature, carbon sources, nitrogen sources and pH. The result indicated that most of them needed higher-temperature, 9 kinds among them grew fast at 30℃, the other 3 grew fast at 25℃. Most of them liked an acidic environment and grew fine when pH was 5～6. The optimum growing conditions were different for various carbon and nitrogen sources.