Abstract: High-quality protein sample preparation is the premise of two-dimensional gel electrophoresis (2-DE). Poplar leaves contain abundant Interfering compounds such as polyphenols, pigments, polysaccharides and so on, which make it difficult to extract proteins. In order to find a method suitable for protein extraction of poplar leaves, the poplar of NI.895 was used as the experimental material. The method of trichloroacetic acid (TCA)-acetone was adopted to extract proteins. Coomassie brilliant blue (CBB) and silver staining were used respectively to detecl the extracted proteins. Good protein patterns were obtained, This paper provides a basis for poplar proteomics research in our lab.