南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (03): 122-124.doi: 10.3969/j.jssn.1000-2006.2007.03.028

• 研究论文 • 上一篇    下一篇

江苏省云台山地蝶类多样性与区系研究

李朝晖1,2,黄成1,虞蔚岩2,王小平2,陈建秀1*,徐善金2   

  1. 1. 南京大学生命科学学院江苏 南京 210093; 2. 南京晓庄学院生命科学系, 江苏 南京 211171
  • 出版日期:2007-06-18 发布日期:2007-06-18

Study on the Diversity and Fauna of Butterflies in Yuntai Low Mountain, Jiangsu Province

LI Zhao-hui1,2, HUANG Cheng1, YU Wei-yan2, WANG Xiao-ping2, CHEN Jian-xiu1*, XU Shan-jin2   

  1. 1. School of Life Science Nanjing University, Nanjing 210093. China; 2. Department of Life Science Naixjing Xiaozhuang University, Nanjing 211171, China
  • Online:2007-06-18 Published:2007-06-18

摘要: <正>根据松材线虫核糖体DNA内部转录区设计了一对特异引物F11/R11与探针TaqMan-11,构成松材线虫实时PCR检测方法。采用该检测方法对分离自枯死松树体内的松材线虫、拟松材线虫、大核滑刃线虫、霍夫曼尼伞滑刃线虫和长尾属线虫进行检测。结果显示,所有松材线虫株系都能够检测到荧光信号,而拟松材线虫、大核滑刃线虫、霍夫曼尼伞滑刃线虫、长尾属线虫以及对照均没有检测到荧光信号。表明探针Taq-Man-11与引物组合对松材线虫具有很强的特异性。此外,利用检测方法,在10μL的反应体系中,最少可以检测到0.005pg的松材线虫DNA含量。实时PCR也可以成功地检测出单条松材线虫。

Abstract: Based on the identification of about 1000 specimens of butterflies collected in Yun7 tai low mountain, the diversity and fauna of butterflies of Yuntai low mountain are concluded: 76 species of butterflies are classified in 7 families and 54 genus. These are Papilionidae 5 genus and 11species, Parnassiidae 1genus and 1species, Pieridae 5 genus and 6 species, Satyridae 7 genus and 12 species, Nymphalidae 15 genus and 18 species, Lycaenidae 10 genus and 15 species, Hesperiidae 11genus and 13 species. The result of a series analysis shows that the percentages of Oriental species, Palearctic species and widely distributive species are 35.53%, 18.42%, 46.05%, respectively. According to the comparison of the similarity, the fauna composition of butterflies is most kindred between Yuntai low mountain and Ningzhen low mountain in Jiangsu Province. Because of the influence from marine climate, the fauna composition of butterflies in Yuntai low mountain is very different from Xiangshan low mountain, so we can see that regional environment impact on the fauna composition most.

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