Abstract: Effects of dilution rates, influent sugar concentration and initial yeasts concentration on single-stage continuous fermentation were investigated. The results showed that dilution rates had significant influence on continuous fermentation. When influent sugar concentration was 45g/L, continuous fermentation arrived at a satisfactory purpose at dilution rates 0.04～0.06h-1, ethanol yield for 0.370～0.372g/g and ethanol productivity for 0.586～0.754g/(L?h). At dilution rates 0.06h-1, the preferable influent sugar concentration was 45g/L, while ethanol productivity was 0.785g/(L?h). If dilution rates and influent sugar concentration were determinated, initial yeasts concentration had little effects on single-stage continuous fermentation. Though single-stage continuous fermentation for total 288h at two different dilution rates, the steady stage was obtained effects 40h of continuous fermentation, and various parameters held stable for about 104h.