Abstract: The relationship between soils from 6 different parent rocks and the growth of Gleditsia sinensis was studied, using the method of experimental ecology. The results showed that the G. sinensis grew best on the limy dolomite with a 209.3 cm high after three years,but poorly grew on the quartzy sandstone with only a height of 100.6 cm. The difference was 2.08 times, and became more and more obvious with the time change. The principles of growth in diameter and height were consistent, but the extent of change was not as large as the height growth. The difference of height and diameter growth also presented a difference of biomass on these soils. The biomass of G. sinensis at three years old reached 382.61g on the limy dolomite, and the poor was only 170.69g on the quartzy sandstone. It showed a difference of 2.2 times. This result provided the theory basis for forest vegetation restoration and tree selection in Karst region of Guizhou province.