兔耳风属3个近缘种ISSR分子鉴别

温强,叶金山,于宏,江香梅

南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (03) : 139-141.

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南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (03) : 139-141. DOI: 10.3969/j.jssn.1000-2006.2008.03.033
研究简报

兔耳风属3个近缘种ISSR分子鉴别

  • 温强,叶金山,于宏,江香梅
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ISSR molecular identification for three species in Ainsliaea

  • WEN Qiang, YE Jin-shan, YU Hong, JIANG Xiang-mei
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摘要

<正>以稻草为原料,利用自制的炭化设备制备稻草炭和稻草醋液,研究炭化温度对稻草炭、稻草醋液以及稻草燃气得率的影响,采用元素分析仪、电感耦合等离子直读光谱仪对稻草炭的元素组成进行了测试与分析。结果表明:稻草炭和稻草燃气的得率随炭化终点温度升高而下降,稻草醋液的得率则随炭化终点温度的升高而升高。稻草炭富含作物生长必需的氮、磷、钾等多种营养元素,其中碳、氮、磷、钾含量分别为3.70×104、8.18×104、3.96×103、3.10×104mg/kg。研究了稻草炭添加量对小白菜生长量的影响,结果表明:当稻草炭的添加量占土壤总量的8%时,小白菜生长量提高较大,增幅为20.87%。

Abstract

Three difficultly-divisional species, i. e., Ainsliaea fragrans, A. macroclinidioides and A. rubrifolia were studied using the ISSR molecular identification system. Twenty primers were screened out from 100 ISSR UBC. They could generate reproducible polymorphic fragments. The particular bands were expanded to make the ISSR fingerprint by primer UBC807, UBC826, UBC834, UBC843, UBC853, and UBC895. It could be used to discriminate three species. Furthermore, according to the alike coefficient UPGMA showed that the re]ative relation of A. fragrans and A. rubrifolia was closer than that of A. fragrans and A. macroclinidioide. The research could be responded for the conservancy and development of the chinese herbal medicine resonrce, and providing a technical support of building up the stable qnality evaluation system of chinese herbal medicine.

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温强,叶金山,于宏,江香梅. 兔耳风属3个近缘种ISSR分子鉴别[J]. 南京林业大学学报(自然科学版). 2008, 32(03): 139-141 https://doi.org/10.3969/j.jssn.1000-2006.2008.03.033
WEN Qiang, YE Jin-shan, YU Hong, JIANG Xiang-mei. ISSR molecular identification for three species in Ainsliaea[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2008, 32(03): 139-141 https://doi.org/10.3969/j.jssn.1000-2006.2008.03.033
中图分类号: S338    Q943   

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