用双向电泳研究里氏木霉在4种 碳源条件下分泌组中纤维降解酶的分布情况。结果表明:在以甘油或葡萄糖为碳源时仅CBHⅡ、AxeI和BXL 3种纤维降解酶有微量表达。在以纤 维二糖为碳源时检测到较高表达水平的CBHⅠ、CBHⅡ、EGⅠ、EGⅢ、BXL、AxeⅠ和AglⅠ。纤维素纸浆可诱导CBHⅠ、CBHⅡ、EGⅠ、EGⅡ、EGⅢ 、EGⅣ、ManⅠ、ManⅡ、AxeⅠ、BXL和AglⅠ等11种纤维降解酶的大量表达,表达量总和占总分泌组的61％。纤维素诱导的纤维降解酶组分情况 和纤维二糖条件相似,但表达量和酶活性都有显著上升。
Abstract
Two dimensional electrophoresis was used to investigate the expression levels of extracellular cellulolytic enzymes of Trichoderma reesei on different carbon sources. The results showed that very low expression levels of CBHⅡ, AxeI and BXL were detected when glucose or glycerol was employed as carbon source. Moderate expression of CBHⅠ, CBHⅡ, EGⅠ, EGⅢ, BXL, AxeI and AglⅠ was observed when cellobiose was used as carbon source. Cellulose induced abundant expression of CBHⅠ，CBHⅡ，EGⅠ，EGⅡ, EGⅢ, EGIV, ManI, ManII, AxeI, BXL and AglⅠ, which accounted for 61％of the secretome. There was some consistency in cellulase composition between the map of cellulose and cellobiose condition, but higher expression levels and enzyme activities were observed when it was induced by cellulose.
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基金
收稿日期：2007-12-07修回日期：2008-04-29 基金项目：国家重点基础研究发展计划项目(2003CB716000,2007CB707801)；国家自然科学基金重点资助项目(20336010) 作者简介：柳常青(1982—),硕士生。*严明（通讯作者）,副教授,研究方向为生物信息学。Email:yanming@njut.edu.cn 引文格式：柳常青,许琳,欧阳嘉,等.不同碳源条件下里氏木霉纤维降解酶的蛋白组解析［J］.南京林业大学学报：自然科学版，2009,33 (1):9296.
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