为了从分子水平上探究外源激素对楸子(Malus prunifolia)插穗中可溶性蛋白组成和内源激素含量变化的影响,利用SDSPAGE电泳技术和酶联免疫吸附分析法,研究了楸子插穗切口处的可溶性蛋白质变化和激素变化与生根的关系。结果表明:分子质量为83、72、65、52、43、39、31、28、26、22 ku的可溶性蛋白质等与生根有关。其中,分子质量为65、39、26、22 ku的蛋白是调控蛋白,当根原基分化时存在,不定根形成后即消失;分子质量为52、43、31、28 ku的蛋白是伴随不定根产生而新形成的蛋白，52 ku的蛋白是一种阻碍细胞分化的蛋白,对生根不利,其余蛋白可促进楸子插穗的生根。内源激素ZR在不定根产生过程中始终维持较高水平,而空白对照中这种趋势不明显。IAA不起主要作用,只起协同作用。GA在根原基分化时期起重要作用并与28 ku蛋白质合成有关。经过处理的插穗中ABA的含量均低于空白对照。说明不同外源激素的处理影响楸子插穗内部激素含量的变化,进而通过调控蛋白质的合成影响蛋白质的种类、数量以及出现时间的变化。

Malus prunifolia Borkh is a kind of nice arbor which holds flower and fruit ornamental merits, whose market needs increase year by year. How to clone mass saplings of M.prunifolia is an urgent issue in its industry. For years, some rooting traits of Malus spp. have been explored at cell level, such as cutting soil material, exogenous hormones, cutting season and other factors’ effects on the abilities of taking roots, as well as the anatomical characters on the rooting of the cuttings. However, the studies on changes of soluble proteins in the process of striking root of M.prunifolia are still not reported. Therefore, to explore this matter helps to establish theoretical basis for cloning mass saplings of M.prunifolia. By applying the techniques of SDS-PAGE & ELISA, this paper studied and explored the relationship between rooting and the variances of both soluble proteins and endogenous plant hormones in the process of striking root cutting of M.prunifolia. The results showed that: during the cutting procedure, the soluble proteins of 83, 72, 65, 52, 43, 39, 31, 28, 26, 22 ku were related to rooting. The soluble proteins in 65, 39, 26, 22 ku existed when the adventitious roots′ primordium differentiated. Furthermore, they disappeared when the cuttings generated adventitious roots. While rooting was in proceeding, some new kinds of soluble proteins like 43, 31, 28 ku that were good for promoting new adventitious roots generated. It seemed that another soluble protein 52 ku in striking roots had some disadvantages to the adventitious root primordium differentiation. Moreover, in the B treatment, soluble proteins 83 ku & 72 ku emerged which had some advantages to both the quantities and qualities of adventitious roots. During the process of striking root, endogenous plant hormones ZR under A & B treatment kept at a high level all the time, whereas CK was relatively low. IAA played a concurrent role other than magistral ones. GA played an important role in adventitious roots’ primordium differentiating and it correlated with the synthesis of 28 ku soluble protein. ABA content in treated cuttings was lower than that of CK. Among them, cuttings under B treatment held lowest record. The conclusion is that different exogenous hormone treatments affect the variances of endogenous hormones of the cuttings of M.prunifolia. Furthermore, they also influenced the types, quantities and arisen time of soluble proteins in the cuttings of M.prunifolia.