Abstract: As one of the good ornamental bamboo species, Menstruocalamus sichuanensis has been flowering in the bamboo garden of Nanjing Forestry University in recent years. The research on the megasporogenesis, microsporogenesis and the development of their female and male gametophyte in M.sichuanensis were made in this paper. The anther wall was consisted of four layers, they were epidermis, endothecium, middle layer and tapetum. The development of anther wall belonged to the monocotyledonous type. The tapetum was typical glandular type. The cytokinesis was successive and the microspore tetrads were bilaterally. Mature pollen grains were mostly twocelled with one germinal aperture. The simple ovary consists of two carpals. Single ovule with parietal placenta was anatropous, double integument and tenuinucellate. The megasporocyte underwent meiotic division to form four megaspores arranged in a line, and the micropylar megaspore was the functional one. The formation of the embryo was polygonum type.