南京林业大学学报(自然科学版) ›› 2009, Vol. 33 ›› Issue (04): 8-12.doi: 10.3969/j.jssn.1000-2006.2009.04.002

• 研究论文 • 上一篇    下一篇

杨树抗杨四瘿螨相关基因的表达分析

张萍,潘惠新,黄敏仁*,王明庥   

  1. 南京林业大学,江苏省杨树种质创新与品种改良重点实验室, 江苏南京210037
  • 出版日期:2009-08-18 发布日期:2009-08-18
  • 基金资助:
    收稿日期:2008-12-29修回日期:2009-05-07基金项目:国家“十一五”科技支撑计划(2006BAD01A1503);国家高技术研究发展计划(2006AA100109)作者简介:张萍(1980—),博士生。*黄敏仁(通讯作者),教授,研究方向为林木遗传育种。Email: mrhuang@njfu.com.cn。引文格式:张萍,潘惠新,黄敏仁,等. 杨树抗杨四瘿螨相关基因的表达分析[J]. 南京林业大学学报:自然科学版,2009,33(4):8-12.

Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)

ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu   

  1. Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China
  • Online:2009-08-18 Published:2009-08-18

摘要: 采用抑制性消减杂交(suppression subtractive hybridization,SSH)技术,在构建杨四瘿螨诱导差异表达的抑制消减cDNA文库的基础上,通过RTPCR技术对候选基因进行了验证,其中有6条差异表达候选基因在4个时间点表达量有明显的增强。运用RACE(Rapid Amplification of cDNA Ends)技术克隆获得了一段全长2 053 bp的cDNA克隆,命名为PtWRKY,编码588个氨基酸。

Abstract: A suppression subtractive hybridization(SSH) cDNA library was constructed with Populus deltoides induced by Tetra lobulifera(Keifer). Realtime PCR was performed to confirm the candidate genes. In this study, six differentially expressed genes were found to be upregulated. Subsequently, the fulllong cDNA sequence about 2 053 bp was cloned by the RACE(Rapid Amplification of cDNA Ends) and named PtWRKY, which encod 588 amino acids.

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