为研究内源多胺对菌根化马尾松植株抗旱能力的影响，以分别接种4种外生菌根真菌及其混合菌的5种菌根化马尾松小苗为材料，通过添加ADC竞争性抑制剂D精氨酸(DArg)进行室内模拟自然干旱胁迫处理，分析了各处理植株的萎蔫度、针叶中腐胺(Put)、亚精胺(Spd)和精胺(Spm)含量的变化及其与抗旱能力的关系。结果显示：未施加DArg的处理中，各植株的抗旱能力依次为接种混合菌根菌的植株较高、紫金蜡蘑(La)菌根化植株次之、CK植株较低，而DArg处理的各植株抗旱能力发生了逆转，依次为CK植株较高、La菌根化植株次之、接种混合菌根菌的植株较低。分析表明菌根化马尾松植株Put合成主要依赖以LArg为前体的途径，并且向Spd和Spm的转化效率较CK植株高；维持较高Spm含量、(Spd+Spm)/Put值，是菌根化马尾松植株抗旱的重要原因之一。
Abstract
We studied the drought resistance of mycorrhizal Pinus massoniana caused by endogenous polyamines following a drought stress in the greenhouse with the treatment of DArg. The results showed that, in drought stress without treatment of DArg, the strength of drought resistance is in the order of P.massoniana inoculated by mixed fungi, inoculated by La and control plants. On the contrary, P.massoniana dealt with DArg and drought stress had taken place reversely. Mycorrhizal P.massoniana can maintain high content of Spm and high (Spd+Spm)/Put value, in which the biosynthetic of Put relies mainly on LArg precursor, and its conversion to Spm and Spd is more efficient. Based on the results, we conclude that ectomycorrhiza can adjust the biosynthetic and conversion of Put, alter the ratio of different polyamines, and then enhance the drought resistance of mycorrhizal P.massoniana.
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基金
收稿日期：2008-12-17修回日期：2009-03-12基金项目：国家自然科学基金资助项目(30571471)；国家“十一五”科技支撑计划(2006BAD08A1002)作者简介：徐超(1979—)，博士生。*吴小芹(通讯作者)，教授，研究方向为森林保护学。Email: xqwu@njfu.com.cn。引文格式：徐超,吴小芹,张红岩. D精氨酸对菌根化马尾松植株内源多胺和抗旱能力的影响[J]. 南京林业大学学报:自然科学版,2009,33(4):19-23.
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