赤松离体培养植株再生体系的建立

朱丽华; 吴小芹; 戴培培; 张红岩

doi:10.3969/j.jssn.1000-2006.2010.02.003

赤松离体培养植株再生体系的建立

朱丽华,,吴小芹,*,戴培培,,张红岩,

南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (02) : 11-14.

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南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (02) : 11-14. DOI: 10.3969/j.jssn.1000-2006.2010.02.003

研究论文

赤松离体培养植株再生体系的建立

朱丽华¹,²,吴小芹¹,²*,戴培培¹,²,张红岩¹,²

作者信息 +

In vitro plantlet regeneration from seedling explants of Pinus densiflora

ZHU Lihua¹,², WU Xiaoqin¹,²*, DAI Peipei¹,², ZHANG Hongyan¹,²

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文章历史 +

摘要

以无菌条件下萌发21~28 d的赤松(Pinus densiflora)幼苗子叶-胚轴材料为起始外植体建立了植株再生体系。结果表明:在添加4 mg/L 6-BA和0.05 mg/L NAA的GD培养基上培养5周可诱导丛生芽形成，无6-BA但添加0.1 mg/L NAA的DCR培养基促进了芽的进一步生长，DCR培养基中添加0.5~1.0 g/L活性炭促进了芽的伸长，在添加2 mg/L 6-BA和0.1~0.2 mg/L NAA的GD培养基上丛生芽大量增殖。伸长的丛生芽接种在附加0.2 mg/L NAA的1/2 GD培养基中培养4周后，不定根发生率达68.4%，转移至无激素但添加0.5 g/L活性炭的1/2 GD中不定根迅速伸长。将完整的再生植株移栽于蛭石-珍珠岩-河沙等比混合的基质中，12周后成活率约为60%。

Abstract

A plantlet regeneration protocol was developed for P.densiflora by using Cotyledonhypocotyl explants from 21—28 days old aseptically grown seedlings. The explants were cultured on a GD medium containing 4 mg/L 6BA (6benzylaminopurine) and 0.05 mg/L NAA (αnaphthylacetic acid) for 5 weeks to obtain bud induction. Bud growth was enhanced by the elimination of 6BA and the inclusion of 0.1 mg/L NAA in DCR medium. Shoots elongation was achieved on DCR medium supplemented with 0.5—1.0 g/L activated charcoal (AC). Multiple shoots in subcultures were proliferated on GD medium with 2 mg/L BAP and 0.2 mg/L NAA. Root primordia were induced in 68.4% of shoots after 4 weeks of culture on 1/2 GD medium containing 0.2 mg/L NAA. Roots elongated rapidly after the transmission of rooted plantlets to 1/2 GD medium with 0.5 g/L AC. The rooted plantlets were successfully transferred to cups containing a mixture of vermiculiteperlitesand. The surviving rate was approximate 60% after 12 weeks in the greenhouse.

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朱丽华,,吴小芹,*,戴培培,,张红岩,. 赤松离体培养植株再生体系的建立[J]. 南京林业大学学报（自然科学版）. 2010, 34(02): 11-14 https://doi.org/10.3969/j.jssn.1000-2006.2010.02.003

ZHU Lihua,, WU Xiaoqin,*, DAI Peipei,, ZHANG Hongyan,. In vitro plantlet regeneration from seedling explants of Pinus densiflora[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2010, 34(02): 11-14 https://doi.org/10.3969/j.jssn.1000-2006.2010.02.003

中图分类号： S718 Q948

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基金

收稿日期：2009-07-07修回日期：2009-10-22基金项目：“十一五”国家科技支撑计划(2006BAD08A19122)作者简介：朱丽华(1970—)，博士后。吴小芹(通信作者)，教授。Email: xqwu@njfu. com.cn。引文格式：朱丽华,吴小芹,戴培培,等. 赤松离体培养植株再生体系的建立[J]. 南京林业大学学报:自然科学版,2010,34(2):11-14.