南京林业大学学报(自然科学版) ›› 2010, Vol. 34 ›› Issue (02): 15-18.doi: 10.3969/j.jssn.1000-2006.2010.02.004

• 研究论文 • 上一篇    下一篇

楸树体细胞胚胎发生的研究

江荣翠,彭方仁*,谭鹏鹏,郝明灼   

  1. 南京林业大学森林资源与环境学院,江苏南京210037
  • 出版日期:2010-05-14 发布日期:2010-05-14
  • 基金资助:
    收稿日期:2009-05-22修回日期:2009-10-26基金项目:“十一五”国家科技支撑计划(2006BAD24B08);江苏省高技术项目(BG2006319)作者简介:江荣翠(1982—),博士生。*彭方仁(通信作者),教授。Email: frpeng@njfu.edu.cn。引文格式:江荣翠,彭方仁,谭鹏鹏,等. 楸树体细胞胚胎发生的研究[J]. 南京林业大学学报:自然科学版,2010,34(2):15-18.

Study on somatic embryogenesis of Catalpa bungei

JIANG Rongcui, PENG Fangren*, TAN Pengpeng, HAO Mingzhuo   

  1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2010-05-14 Published:2010-05-14

摘要: 以楸树近成熟种子培养无菌苗,以无菌苗子叶和下胚轴为外植体进行体细胞胚胎发生研究,观察了体细胞胚胎的发育过程,分析了不同生长调节物质对楸树体胚发生的影响。结果表明:各培养基均能诱导出愈伤组织;1/2MS+0.01 mg/L NAA+1.00 mg/L 6-BA可调整愈伤至胚性愈伤,是体细胞胚胎诱导频率最高的培养基;带少量愈伤的体胚可在1/2MS0和1/2MS+1.00 mg/L 6-BA+0.01 mg/L NAA的培养基上增殖,且增殖系数较大。

Abstract: The process of somatic embryogenesis (SE) of Catalpa bungei and the effects of different growth regulators on SE were studied with explants of cotyledons and hypocotyls of seedlings. The seedlings were cultured from near matured seeds of C.bungei. The results showed that callus could be induced on each medium in this paper. Callus could be transferred to embryogenic callus on the medium of 1/2MS+0.01 mg/L NAA+1.00 mg/L 6-BA, on which the SE were induced with the highest frequency. The SE were able to proliferate on the medium of 1/2MS0 and 1/2MS+1.00 mg/L 6-BA+0.01 mg/L NAA with high multiplication coefficient.

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