对北京十三陵苗圃北京蟒山、山西孝义和甘肃两当3个种源的8年生白皮松1年生针叶进行了电阻抗图 谱(EIS)分析，测定各种源不经冷冻处理针叶的EIS参数，以及超氧化物歧化酶(SOD)活性、过氧化物酶 (POD)活性、丙二醛(MDA)含量、可溶性蛋白质、脯氨酸、叶绿素和干物质含量等生理指标的变化；分析EIS 参数和生理指标之间的相关性；用褐变法测定了针叶的抗寒性。结果表明：(1)针叶的EIS参数和生理指标 随着抗寒性的提高发生变化，不同种源的EIS参数和某些生理指标在不同抗寒锻炼阶段，尤其在抗寒锻炼初 期存在显著差异，并与不同种源抗寒性差异吻合；(2)EIS参数细胞膜时间恒量τm和胞外电阻re与针叶的叶 绿素含量存在极显著负相关(r分别为-0.91和-0.68)，β与针叶的干物质含量存在极显著负相关(r=-0.74) ，而胞内电阻ri和re与针叶的干物质含量存在极显著正相关(r分别为0.69和0.58)，β与MDA含量存在极显 著正相关(r=0.68)；(3)β与褐变法求得的抗寒性存在极显著正相关(r=0.85)，ri和re存在极显著负相关(r 分别为-0.79和-0.66)。
Abstract
The electrical impedance spectroscopy (EIS) was analyzed to oneyearold needles of Pinus bungeana Zucc.in an 8year provenance field trial at the Nursery in Beijing Thirteen Tombs with provenances of Mangshan of Beijing, Xiaoyi of Shanxi, and Liangdang of Gansu. The parameters of EIS and the activities of superoxide dismutase (SOD), the activities of peroxidase (POD), the content of malondialdehyde (MDA), the content of soluble protein, the content of proline, the chlorophyll content and the content of dry matter in nonfrost exposed needles were tested with different provenances. The relations between EIS and physiological parameters were analyzed. The frost hardiness (FH) of needles was measured by means of the visual scoring of damage (VSD) after artificial freezing test. The results showed that: (1) Both the EIS parameters and the physiological parameters changed during frost hardening, and the EIS parameters and some physiological parameters differed significantly in certain stages of frost hardening, especially in the early stage, which was in accordance with the differences of FH in different provenances. (2) The EIS parameters membrane time constant τm and extracellular resistance re correlated negatively with the chlorophyll content (the correlation coefficient r were -0.91 and -0.68, respectively), the β correlated negatively with the dry matter content (r=-0.74), while the intracellular resistance ri and re had positive correlation with the dry matter content (r were 0.69 and 0.58, respectively), as well as the β with the MDA content (r=0.68). (3) The β correlated positively with the FH assessed by the VSD method (r=0.85), whereas the ri and re did negatively (r were -0.79 and -0.66, respectively).
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]陈禅友,汪汇东,丁毅. 低温胁迫下长豇豆幼苗可溶性蛋白质和细胞保护酶活性的变化[J]. 园艺学 报,2005,32(5):911-913.
[2]林善枝,李雪平,张志毅. 低温锻炼对毛白杨幼苗抗冻性和总可溶性蛋白质的影响[J]. 林业科学,2002 (11):137-141.
[3]刘慧民,王崑,李奇石,等. 五叶地锦低温处理条件下与抗寒相关的部分生理生化指标的变化规律[J]. 东 北林业大学学报,2003,31(4):74-75.
[4]Greer D H. Electrical impedance and its relationship to frost hardiness in Pinus radiate [J]. New Zealand Journal of Forestry Science, 1983, 13(1): 80-86.
[5]Stout D G. Effect of cold acclimation on bulk tissue electrical impedance.Ⅰ.Measurements with birdsfoot trefoil at subfreezing temperatures[J]. Plant Physiology, 1988, 86: 275-282.
[6]Repo T, Zhang M. Modelling woody plant tissues using a distributed electrical circuit[J]. Journal of Experimental Botany, 1993, 44: 977-982.
[7]Repo T, Zhang G, Ryypp A, et al. The relation between growth cessation and frost hardening in Scots pines of different origins[J]. Trees, 2000, 14: 456-464.
[8]Mancuso S, Rinaldelli E. Response of young mycorrhizal and nonmycorrhizal plants of olive tree (Olea europaea L.) to saline conditions.Ⅱ.Dynamics of electrical impedance parameters of shoots and leaves[J]. Advances in Horticultural Science, 1996, 10: 135-145.
[9]刘晓红,王国栋,张钢. 干旱胁迫下小麦叶片的电阻抗参数与生理参数关系[J]. 西北植物学报,2007,27 (5):970-976.
[10]刘晓红,王国栋,张钢. 外源重金属胁迫对小麦叶片电阻抗参数的影响[J]. 农业环境科学学 报,2007,26(6):2014-2020.
[11]Mancuso S. Seasonal dynamics of electrical impedance parameters in shoots and leaves relate to rooting ability of olive (Olea europaea) cuttings[J]. Tree Physiology, 1998, 19: 95-101.
[12]Zhang G, Ryypp A, Repo T. The electrical impedance spectroscopy of Scots pine needles during cold acclimation[J]. Physiologia Plantarum, 2002, 115: 385-392.
[13]Repo T, Zhang G, Ryypp A, et al. The electrical impedance spectroscopy of Scots pine (Pinus sylvestris L.) shoots in relation to cold acclimation[J]. Journal of Experimental Botany, 2000, 51: 2095-2107.
[14]Vainola A, Repo T. Impedance spectroscopy in frost hardiness evaluation of Rhododendron leaves[J]. Annals of Botony, 2000, 86: 799-805.
[15]Zhang G, Ryypp A, Vapaavuori E, et al. Quantification of additive response and stationarity of frost hardiness by photoperiod and temperature in Scots pine[J]. Canadian Journal of Forest Research, 2003,33: 1772-1784.
[16]许绍惠,边立琪,郭泳,等. 白皮松抗寒性及抗寒育苗技术的研究[J]. 林业科学,1994,30(6):497-505.
[17]Cappiello P E, Dunham S W. Seasonal variation in lowtemperature tolerance of Vaccinium angustifolium Ait[J]. Hort Science, 1994, 29: 302-304.
[18]Burr K E B, Hawkins C D B, L’Hirondelle S J L, et al. Methods for Measuring Cold Hardiness of Conifers[G]//Bigras F J, Colombo S J. Conifer Cold Hardiness. Dordrecht: The Netherlands: Kluwer Academic Publishers, 2001.
[19]Zhang M I N, Repo T, Willison J H M, et al. Electrical impedance analysis in plant tissues: on the biological meaning of ColeCole α in Scots pine needles[J]. European Biophysics Journal, 1995, 24: 99-106.
[20]Repo T, Zhang M, Ryypp A, et al. Effects of freezethaw injury on parameters of distributed electrical circuits of stems and needles of Scots pine seedlings at different stages of acclimation[J]. Journal of Experimental Botany, 1994, 45: 823-833.
[21]李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000.
[22]张宪政. 作物生理研究法[M]. 北京:农业出版社,1992.
[23]张钢,刘民,任元新. 春季白皮松实生苗的脱锻炼与再锻炼[J]. 植物生理学通讯,2005,41(6):761-763.
[24]Repo T, Lappi J. Estimation of standard error of impedanceestimated frost resistance [J]. Scandinavian Journal of Forest Research, 1989, 4: 67-74.
[25]Jin Y H, Tao D L, Du Y J. Freezing tolerance, pigments and SOD of fire conifers in Shenyang[J]. Journal of Integrative plant Biology, 1990, 32(9): 702-706.
[26]Repo T, Oksanen E, Vapaavuori E. Effects of elevated concentrations of ozone and carbon dioxide on the electrical impedance of leaves of silver birch (Betula pendula) clones[J].
基金
收稿日期：2009-07-29修回日期：2009-10-25基金项目：国家自然科学基金项目 (30640035);河北农业大学留学回国人员科研启动基金项目(200406);河北农业大学新兴学科发展基金项目 (200608)作者简介：李亚青(1982—)，硕士。*张钢(通信作者)，教授，博士。Email: zhanggang1210@126.com。引文格式：李亚青,张钢,董胜豪,等. 抗寒锻炼期间影响白皮松针叶电阻抗图谱 参数的生理指标[J]. 南京林业大学学报:自然科学版,2010,34(2):24-30.
PDF(1045089 KB)
