在里氏木霉和黑曲霉液态混合条件下培养纤维素酶，分析两个菌种的接种比和延迟黑曲霉的接种时间对产酶的影响，探讨两个菌种发挥协同作用的最佳条件。结果表明：黑曲霉延迟接种48 h及里氏木霉与黑曲霉接种质量比1∶1时 ，所产纤维素酶的滤纸酶活为1163 μmol/(min·mL)；β-葡萄糖苷酶活为0606 μmol/(min·mL)，β-葡萄糖苷酶活与滤纸酶活比值为0521，比单一里氏木霉产纤维素酶的酶活高，并在后续的酶解效果对比中表现最佳。48 h时的酶解得率为6561 %，高于里氏木霉单一培养时所产纤维素酶的得率5391 %和商品纤维素酶的得率4964 %。说明通过混合发酵，纤维素酶的组分得到了优化。
Abstract
Mixed culture of Trichoderma reesei and Aspergillus niger was investigated to optimize the cellulase components in liquidstate fermentation. A mode of delayed inoculation was applied for cocultivation of T. reesei and A. niger. After cultivation of T. reesei for 48 h, A. niger was inoculated at the 1∶1 inoculum ratio. The βglucosidase activity and filter paper activity of the mixed fungi culture were 0.606 μmol/(min·mL) and 1.163 μmol/(min·mL), respectively, corresponding to a ratio of 0.521, which was higher than that of T. reesei under single cultivation. The yield of enzymatic hydrolysis was 65.61 % by the cocultivated cellulase, which was higher than those of singlecultivated cellulase and commercial cellulase. These results demonstrated that cocultivation of T. reesei and A. niger was suitable for optimization of cellulase components.
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基金
收稿日期：2010-04-29修回日期：2010-12-09 基金项目：国家自然科学基金项目（30871992）；江苏省科技支撑计划（BE2010732）；江苏高校科技创新团队项目作者简介：宋向阳(1965—)，副教授，博士。Email: xiangyangsong@hotmail.com。引文格式：宋向阳，方浩，欧阳嘉,等. 液态混合菌发酵优化纤维素酶组分[J]. 南京林业大学学报:自然科学版,2011,35(2):78-82.
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