Abstract: We studied a new way to determine the effect of different factors on Bursaphelenchus. xylophilus migration behavior using sterile sand tray and agar surface as migration matrixes. We also separated different extracts from the fungus Botrytis cinerea culture medium and used a filter paper method to determine the effects of the extracts on B. xylophilus activity. The results showed that pine wiltaffected wood has a strong attraction effect on B. xylophilus. The results differed from the presumption that the attractant is a volatile substance. The inducing force of B. cinerea toward B. xylophilus is stable while the pine skin lixivium has no obvious inducing effect. As well, B. xylophilus was obviously attracted to different fungi (B. cinerea>Pestalotia>Microzyme>control), i.e. the attraction to B. cinerea is greatest. This difference increases as exposure time increases and also as the concentration of metabolic products increase. Also, agar concentration significantly influenced migration behavior with the attractiveness to B. xylophilus increasing as the agar concentration decreases. The studies made to determine the nature of the attractants showed that the relative attractiveness of the different B. cinerea extracts to B. xylophilus differed according to the separation method used. Also, the active substances mainly existed in the extracellular organic phase (ethyl acetate phase) suggesting that they might be ethanol soluble compounds. With increased separation the attraction efficiency decreased. Based on the results, we conclude that the inducing activity depends upon the synergistic action of these extracts. Acknowledgment: This research was supported by National Natural Science Fundation of China (30470234).