以‘超级红’等唐菖蒲切花品种的花粉为试材,研究了花蕾的开放状态、花粉含水量、不同干燥时间与不同保存温度、预冻方式和解冻方式对唐菖蒲花粉萌发率的影响,同时测定了唐菖蒲4个栽培品种花粉的长期保存效果。结果表明:唐菖蒲花朵刚呈展开状态时的花粉萌发率最高；未干燥花粉和干燥1 h之内花粉萌发率次高。保存温度和含水量对花粉的保存效果影响显著,花粉在干燥0～1 h、-80 ℃温度下保存效果好；0 ℃预冻有利于花粉的低温保存；37 ℃温水化冻处理的花粉萌发率明显高于其他解冻方式。唐菖蒲4个栽培品种花粉的长期保存以-80 ℃低温条件效果较好,保存时间可达1 a以上,0 ℃处理的次之,4 ℃和-20 ℃条件下保存的花粉萌发率较低,且品种间差异显著。
Abstract
Effects of different statuses of flower buds, water content of pollen, different drying times and storage temperatures, prefreezing and thawing methods on germination rate of pollens were investigated. Also the results of longterm preservation were compared among pollens of different cultivars. The results showed that the pollens of just opening flowers perform higher viability. The storage temperature and water content were two important factors affecting the pollen life. The pollens dried 0—1 hour and stored at -80 ℃ have higher viability. Prefreezing at 0 ℃ and the thawing at 37 ℃ water could promote the viability of pollens. The results of longterm storage showed that pollen storage at -80 ℃ were more suitable than other temperatures, pollen storage time would reach one year, followed by storage at 0 ℃, the germinability of pollen stored at 4 ℃ and -20 ℃ was lower. In addition, remarked difference was observed in the viability of stored pollens among different cultivars.
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参考文献
[1]Koopowitz H, O’Neil C. Longterm storage of Gladiolus pollen［J］.Hort Science, 1984, 19(4): 513-514.
[2]Takatsu Y, Kasumi M, Manabe T, et al. Longstorage of pollen of wild Gladiolus species［J］.Bulletin of the Plant Biotechnology Institute Ibaraki Agricultural Center， 2001(4): 51-54.
[3]Rajasekharan P E, Rao T M, Janakiram T, et al. Freeze preservation of Gladiolus pollen［J］. Euphytica, 1994, 80: 105-109.
[4]黄蓓丽,耿兴敏,胡凤荣,等.唐菖蒲花粉离体萌发的液体培养基研究［J］.安徽农业大学学报, 2009, 36 (4):593-597.
[5]尚晓倩.芍药花粉超低温保存研究［D］.北京:北京林业大学,2005.
[6]李广清.山茶花粉超低温保藏研究［D］.北京:北京林业大学,2005.
[7]Matthews F R, Kraus J F. Pollen storage[C]//Franklin C.Pollen Management Handbook. Washington DC:Dep Agric， 1981.
[8]Harrington J F. Seed and pollen storage for conservation of plant gene resources[C]//Frankpi Bennett E. Genetic Pesources in Plant and Their Exploration and ConserVation.OxfordEdinburgh:Blackwell Scientific Publications,1970.
[9]Luza J G, Polito V S. Cryopreservation of English walnut (Juglans regia L.) pollen［J］.Euphytica, 1988, 37:141-148.
[10]王彩虹,李嘉瑞.杏花粉的低温与超低温贮藏研究［J］.莱阳农学院学报,1996,13(2):169-173.
[11]程斐,翟晓巧.豫杂一号泡桐花粉超低温保存技术研究［J］.河南林业科技，2009,29(1):1-3.
[12]Sacks E J, Clair D A S. Cryogenic storage of tomato pollen:effect on fecundity［J］. Hort Science,1996,31(3): 447-448.
[13]张玉进,张兴国,刘佩瑛.魔芋花粉的低温和超低温保存［J］.园艺学报,2000, 27(2):139-140.
[14]张亚利.梅花花粉超低温保存研究及其花粉库的建立［D］.北京:北京林业大学，2007.
[15]陈品良.植物组织培养物的超低温保存［J］.武汉植物学研究, 1989, 7(4):390-398.
[16]陈珊.蜡梅属植物花粉低温与超低温保藏研究［D］.武汉:华中农业大学, 2005.
基金
收稿日期：2010-08-25修回日期：2010-12-17 基金项目：南京林业大学引进人才基金项目(B2007-8) 作者简介：耿兴敏(1976—),副教授,博士。Email:xmgeng@njfu.edu.cn。
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