南京林业大学学报(自然科学版) ›› 2011, Vol. 35 ›› Issue (05): 31-34.doi: 10.3969/j.jssn.1000-2006.2011.05.007

• 研究论文 • 上一篇    下一篇

激素和光照对抗松材线虫病赤松丛生芽离体保存的影响

秦莉,吴小芹*   

  1. 南京林业大学,江苏省有害生物入侵预防与控制重点实验室,江苏南京210037
  • 出版日期:2011-09-30 发布日期:2011-09-30
  • 基金资助:
    收稿日期:2010-07-13修回日期:2010-12-27基金项目:国家林业局林业公益性行业科研专项项目(200704026);国家林业局“948”项目(2011-4-69)作者简介:秦莉(1984—),硕士生。*吴小芹(通信作者),教授。Email: xqwu@njfu.com.cn。

Effects of hormone and light on in vitro preservation of axillary buds of Pinus densiflora resistant to pine wilt disease

QIN Li, WU Xiaoqin*   

  1. Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing 210037, China
  • Online:2011-09-30 Published:2011-09-30

摘要: 为了保存通过抗病性测定表明具优良抗性的抗松材线虫病赤松丛生芽,笔者研究了不同激素和光照处理对其离体保存的影响。结果表明:17 ℃条件下,暗培养不适合抗病赤松丛生芽的离体保存;而在培养基中仅添加10 mg/L ABA或添加02 mg/L 6-BA+ (01~02) mg/L NAA的组合对丛生芽限制生长保存具有较好效果。二者均可使丛生芽离体保存后存活率高于94 %且恢复生长后芽体增殖率为100 %,植株生长健壮。

Abstract: In order to preserve axillary buds of the Pinus densiflora with excellent resistance against Bursaphelenchus xylophilus, the effects of different treatments of phytohormones and light on bud preservation were studied. It showed that dark condition was not favorable to in vitro bud preservation at 17 ℃. On the contrary, addition of 1.0 mg/L ABA or 02 mg/L 6BA plus 0.1-0.2 mg/L NAA under lightdark alteration had a positive effect on the bud preservation. In this condition, survival rate of the axillary buds was 94 % or higher and all survived buds proliferated well.

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