运用响应面分析法研究了柱状假丝酵母脂肪酶去除麦秸中亲脂类抽提物的影响因素。结果表明:在脂肪酶用量、处理温度、pH和处理时间等因素中,脂肪酶用量对亲脂类抽提物的去除影响最为显著,而温度的影响最小。亲脂类抽提物去除率随着脂肪酶用量、酶处理时间的增加而提高,脂肪酶处理过程中适宜温度为46 ℃、pH为7.6的实验条件下,亲脂类抽提物的最大去除率为61.1%。因此,脂肪酶能有效地去除麦秸中亲脂类抽提物,将成为改善麦秸润湿性能的有效手段。
Abstract
The removal efficiency of lipophilic extractives from wheat straw(WS)by Candida cylindracea lipases was investigated using response surface method. The results showed that the lipase dosage was the most significant and the temperature was the least significant among all parameters including lipase dosage, temperature, pH and time during the lipase treatment. The removal rate of lipophilic extractives increased with the lipase dosage increasing and the treatment time prolonging. The optimal temperature and pH were 46℃ and 7.6, respectively. The maximum removing rate of lipophilic extractives reached 61.1% at aforesaid conditions.It was indicated that the lipases can effectively remove the lipophilic extractives from WS,and the lipace treatment was an effective method to improve WS wettability.
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基金
收稿日期:2011-03-30 修回日期:2011-12-05 基金项目:国家自然科学基金项目(30671640); 江苏省高校优势学科建设工程资助项目 第一作者:江华,教授,博士。E-mail: jianghua@njfu.com.cn。引文格式:江华,张洋,王雪飞,等. 柱状假丝酵母脂肪酶去除麦秸中亲脂类抽提物的研究[J]. 南京林业大学学报:自然科学版,2012,36(2):35-41.
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