高等植物脂肪酸去饱和酶及编码基因研究进展

doi:10.3969/j.jssn.1000-2006.2012.02.026

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2012, Vol. 36 ›› Issue (02): 125-132.doi: 10.3969/j.jssn.1000-2006.2012.02.026

高等植物脂肪酸去饱和酶及编码基因研究进展

曹福亮¹,王欢利¹,郁万文¹,程华^1,2

1.南京林业大学森林资源与环境学院,江苏南京,210037; 2.黄冈师范学院生命科学与工程学院,湖北黄冈,438000

出版日期:2012-04-10 发布日期:2012-04-10
基金资助:
收稿日期:2010-11-16 修回日期:2011-12-12 基金项目:国家林业局“948”项目(2008-4-05); 高等学校博士学科点专项科研基金项目(20093204120004; 20113204120007) 第一作者:曹福亮,教授。E-mail: flcao126@126.com。引文格式:曹福亮,王欢利,郁万文,等. 高等植物脂肪酸去饱和酶及编码基因研究进展[J]. 南京林业大学学报:自然科学版,2012,36(2):125-132.

Progress of research on fatty acid desaturase and their coding genes in higher plant

CAO Fuliang¹, WANG Huanli¹, YU Wanwen¹, CHENG Hua^1,2

1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.College of Life Science and Engineering, Huanggang Normal University, Huanggang 438000, China

Online:2012-04-10 Published:2012-04-10

摘要/Abstract

摘要： 生物膜是细胞、细胞器与外界环境连接的界面,是温度胁迫伤害发生的原初位点。细胞膜脂不饱和脂肪酸含量和组分是影响生物膜相变的主要因素,进而影响植物在温度胁迫下的抗性。脂肪酸去饱和酶是脂肪酸代谢过程中的关键酶,调节细胞膜中脂肪酸的含量和组分。笔者根据脂肪酸去饱和酶在催化反应中的先后顺序将其分为3类,分别阐述这3类脂肪酸去饱和酶与温度胁迫下植物适应性的关系,综述了近年来国内外脂肪酸酶去饱和酶及编码基因的克隆、功能鉴定、表达调控等方面的研究进展,对应用基因工程技术培育出温度抗性较强植物品种的研究进行了展望。

Abstract: Biologic membrane is a structural interface between plant cell, organelle and surrounding environment. Biologic membrane was regarded as the initial position which suffered under temperature stress. The contents and compositions of unsaturated fatty acid in plant membrane is the main factor that affects the biologic membrane transformation, and then influence the resistance to temperature stress. Fatty acid desaturase are the key enzymes during the synthesis of fatty acid, which regulate the contents and compositions of fatty acid in plant membrane. Fatty acid desaturase were classified into three groups according to the order of catalysis in this paper. The authors illustrated the relationship between these groups of fatty acid desaturase and temperature stress resistance. Overviewed the research advances on gene cloning, function identification and expression regulation of fatty acid desaturase.

中图分类号:

Q949

曹福亮,王欢利,郁万文,等. 高等植物脂肪酸去饱和酶及编码基因研究进展[J]. 南京林业大学学报（自然科学版）, 2012, 36(02): 125-132.

CAO Fuliang, WANG Huanli, YU Wanwen, CHENG Hua. Progress of research on fatty acid desaturase and their coding genes in higher plant[J].Journal of Nanjing Forestry University (Natural Science Edition), 2012, 36(02): 125-132.DOI: 10.3969/j.jssn.1000-2006.2012.02.026.

参考文献

[1] Lyons J M. Chilling injury in plants [J]. Ann Rev Plant Physiol, 1973, 24: 445-466.
[2] 徐燕,薛立,屈明.植物抗寒性的生理生态学机制研究进展[J].林业科学,2007,43(4):88-94.
[3] Gounaris K, Brain A P R, Quinn P J, et al. Structural reorganization of chloroplast thylakoid membranes in response to heat stress [J]. Biochim Biophys Acta, 1984, 766: 198-208.
[4] Gounaris K, Brain A P R, Quinn P J, et al. Structural and functional changes associated with heat-induced phase-separation of non-bilayer lipids in chloroplast thylakoid membranes [J]. FEBS Lett, 1983, 153: 47-52.
[5] 翟中和,王喜忠,丁明孝.细胞生物学[M].北京:高等教育出版社,2000.
[6] 林萍,齐力旺,汪阳东,等.植物抗寒工程中脂肪酸去饱和酶研究进展[J].分子植物育种,2006,4(3):404-410.
[7] Murata N, Los D A. Membrane fluidity and temperature perception [J]. Plant Physiol, 1997, 115(3): 875-879.
[8] Nishida I, Murata N. Chilling sensitivity in plants and cynobacteria:the crucial contribution of membrane lipids [J]. Annu RevPlant Physiol Plant Mol Biol, 1996, 47: 541-568.
[9] Somerville C. Direct tests of the role of membrane lipid composition in low-temperature-induced photoinhibition and chilling sensitivity in plants and cyanobacteria [J]. Proc Natl Acad Sci USA, 1995, 92(14): 6215-6218.
[10] 费云标,黄涛,舒念涛,等.植物抗寒冻的分子遗传与基因工程[J].生物工程进展,1996,16:15-16.
[11] 叶华勋.播娘蒿硬脂酸脱饱和酶的基因克隆及其功能研究[D].成都:四川大学,2007.
[12] 邬贤梦,官春云,李栒.油菜脂肪酸品质改良的研究进展[J].作物研究,2003,17(3):152-158.
[13] 戴晓峰,肖玲,武玉花,等.植物脂肪酸去饱和酶及其编码基因研究进展[J].植物学通报,2007,24(1):105-113.
[14] Dyer J M, Mullen R T. Immunocytological localization of two plant fatty acid desaturases in the endoplasmic reticulum [J]. FEBS Lett, 2001, 494: 44-47.
[15] Ohlrogge J, Browse J. Lipid biosynthesis [J]. Plant Cell, 1995, 7: 957-970.
[16] 曾硕士,江黎明,元冬娟,等.脂肪酸去饱和酶的研究进展[J].生命科学,2008,20(5):816-820.
[17] 张羽航,鲍时翔,郑学勤,等.脂肪酸脱饱和酶的研究进展[J].生物技术通报,1998(4):1-9.
[18] Lindqvist Y, Huang W, Schneider G, et al. Crystal structure of delta 9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins [J]. EMBOJ, 1996, 15: 4081-4092.
[19] Kodama H, Horiguchi G, Nishiuchi T, et al. Fatty acid desaturation during chilling acclimation is one of the factors involved in conferring low-temperature tolerance to young tobacco leaves [J]. Plant Physiol, 1995, 107(4): 1177-1185.
[20] Luo T, Peng S M, Deng W Y, et al. Characterization of a new stearoyl-acyl carrier protein desaturase gene from Jatropha curcas [J]. Biotechnology Letters, 2006, 28: 657-662.
[21] Madil L, Wang X J, Kobiler L, et al. Stress on avocado fruits regulates Δ9-stearoyl ACP desaturase expression,fatty acid composition, antifungal diene level and resistance to Colletotrichum gloeosporioides attack [J]. Physiol Mol Plant Pathol, 2003, 62: 277-282.
[22] Luo T, Deng W Y, Zeng J, et al. Cloning and characterization of a stearoyl-acyl carrier protein desaturase gene from Cinnamomum longepaniculatum [J]. Plant Mol Biol Rep, 2009, 27: 13-19.
[23] 范妙华,李纪元,范正琪,等.植物脂肪酸脱饱和酶特性及转基因研究进展[J].生物技术通报,2007(6):16-20,36.
[24] Knutzon D S, Thompson G A, Radke S E, et al. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene [J]. Proc Natl Acad Sci USA, 1992, 89: 2624-2628.
[25] Liu Q, Singh S P, Green A G. High-Stearic and High-Oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing[J]. Plant Physiol, 2002, 129:1732-1743.
[26] Zaborowska Z, Starzycki M, Femiak I, et al. Yellow lupine gene encoding stearoyl-ACP desaturase-organization, expression and potential application[J]. Acta Biochim, 2002, 49: 29-42.
[27] Byfield G E, Upchurch R G. Effect of temperature on delta-9 stearoyl-ACP and microsomal omega-6 desaturase gene expression and fatty acid content in developing soybean seeds [J]. Crop Sci, 2007, 47(4): 1698-1704.
[28] Vega S E, Delrio A H, Bamberg J B, et al. Evidence for the up-regulation of stearoyl-ACP(Δ9)desaturase gene expression during cold acclimation [J]. Am J Potato Res, 2004, 81(2): 125-135.
[29] Lightner J, Wu J, Browse J. A mutant of Arabidopsis with increased level of stearic acid[J]. Plant Physiol, 1994, 106:1443-1451.
[30] Ekharde, Ziegler L J, Filer J R.现代营养学[M].北京:人民卫生出版社,1998.
[31] 石东乔,周奕华,陈正华.植物脂肪酸调控基因工程研究[J].生命科学,2002,14(5):291-295,317.
[32] Watanabe K, Oura T, Sakai H, et al. Yeast Δ12 fatty acid desaturase:gene cloning,expression,and function [J]. Biosci Biotechnol Biochem, 2004, 68(3): 721-727.
[33] 周洲.转脂肪酸去饱和酶基因PtFAD2和PtFAD3银腺杨84K的抗寒性研究[D].北京:中国林业科学研究院,2007.
[34] Matteucci M, D’Angeili S, Errico S, et al. Cold affects the transcription of fatty acid desaturases and oil quality in the fruit of Olea europaea L. genotypes with different cold hardiness[J]. Exp Bot, 2011, 62(10): 3403-3420.
[35] Zhang J T, Zhu J Q, Zhu Q, et al. Fatty acid desaturase-6(Fad6)is required for salt tolerance in Arabidopsis thaliana [J]. Biochem and Biophys Res Commu, 2009, 390: 469-474.
[36] Los D A, Ray M K, Murata N. Differences in the control of the temperature-dependent expression of four genes for desaturases in Synechocystis sp. PCC 6803 [J]. Mol Microbiol, 1997, 25(6): 1167-1175.
[37] Gombos Z, Wada H, Murata N. Unsaturation of fatty acids in membrane-lipids enhances tolerance of the cyanobacterium Synechocystis PCC6803 to low-temperature photoinhibition [J]. Proc Natl Acad Sci U S A, 1992, 89(20): 9959-9063.
[38] Lu Y D, Chi X Y, Li Z X, et al. Isolation and characterization of a stress-dependent plastidial delta 12 fatty acid desaturase from the Antarctic microalga Chlorella vulgaris NJ-7[J]. Lipids 2010, 45(2): 179-187.
[39] 高岩,郭东林,郭长虹.三烯脂肪酸在高等植物逆境胁迫应答中的作用[J].分子植物育种,2010,8(2):365-369.
[40] 刘训言,孟庆伟,李滨.植物ω-3脂肪酸去饱和酶的研究进展[J].细胞生物学杂志,2004,26(1):34-38.
[41] 于超.温度胁迫下番茄内质网ω-3脂肪酸去饱和酶基因的表达和功能分析[D].泰安:山东农业大学,2009.
[42] Matsuda O, Watanabe C, Iba K. Hormonal regulation of tissue-specific ectopic expression of an Arabidopsis endoplasmic reticulum-type omega-3 fatty acid desaturase(FAD3)gene [J]. Planta, 2001, 213: 833-840.
[43] Shimada T, Wakita Y, Otani M, et al. Modification of fatty acid composition in rice plants by transformation with a tobacco microsomal-3 fatty acid desaturase gene(NtFAD3)[J]. Plant Biotechno Lett, 2000, 17: 43-48.
[44] Horiguchi G, Fuse T, Kawakami N, et al. Temperature-dependent translational regulation of the ER omega-3 fatty acid desaturase gene in wheat root tips [J]. Plant J, 2000, 24(6): 805-813.
[45] Kodama H, Nishiuchi T, Seo S, et al. Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid omega-3 fatty acid desaturase gene [J]. Plant Sci, 2000, 155: 153-160.
[46] 周洲,李永丽.毛白杨亚油酸去饱和酶基因的克隆与表达模式分析[J].西北林学院学报,2009,24(5):61-65.
[47] Yu C, Wang H S, Yang S, et al. Overexpression of endoplasmic reticulum omega-3 fatty acid desaturase gene improves chilling tolerance in tomato [J]. Plant Physiol Biochem, 2009, 47(11-12): 1102-1112.
[48] Dominguez T, Hernandez M L, Pennycooke J C, et al. Increasing omega-3 desaturase expression in tomato results in altered aroma profile and enhanced resistance to cold stress[J].Plant Physio, 2010, 153(2): 655-665.
[49] Matsuda O, Sakamoto H, Hashimoto T, et al. A temperature-sensitive mechanism that regulates post-translational stability of a plastidial omega-3 fatty acid desaturase(FAD8)in Arabidopsis leaf tissues [J]. J Biol Chem, 2005, 280(5): 3597-3604.
[50] Horiguchi G, Iwakawa H, Kodama H, et al. Expression of a gene for plastid ω-3 fatty acid desaturase and changes in lipid and fatty acid compositionsin light and dark-grown wheat leaves [J]. Physiol Plant, 1996, 96: 275-283.
[51] Murakami Y, Tsuyama M, Kobayashi Y, et al. Trienoic fatty acids and plant tolerance of high temperature [J]. Science, 2000, 287(5452): 476-479.
[52] Iba K, Gibson S, Nishiuchi T, et al. A gene encoding a chloroplast omega-3 fatty acid desaturase complements alterations in fatty acid desaturation and chloroplast copy number of the FAD7 mutant of Arabidopsis thaliana [J]. J Biol Chem, 1993, 268(32): 24099-24105.
[53] Vijayan P, Browse J. Photoinhibition in mutants of Arabidopsis deficient in thylakoid unsaturation [J]. Plant Physiol, 2002, 129(2): 876-885.
[54] Kodama H, Hamada T, Horiguchi G, et al. Genetic enhancement of cold tolerance by expression of a gene for chloroplast ω-3 fatty acid desaturase in transgenic tobacco [J]. Plant Physiol Bioch, 1994, 105(2): 601-605.
[55] 刘训言.番茄叶绿体ω-3脂肪酸去饱和酶基因(LeFAD7)的克隆及其在温度逆境下的功能分析[D].泰安:山东农业大学,2006.
[56] Berberich T, Harada M, Sugawara K, et al. Two maize genes encoding omega-3 fatty acid desaturase and their differential expression to temperature [J]. Plant Mol Biol, 1998, 36(2): 297-306.
[57] Wang J, Ming F, Pittman J, et al. Characterization of a rice(Oryza sativa L.)gene encoding a temperature-dependent chloroplast ω-3 fatty acid desaturase [J]. Biochem Biophys Res Commun, 2006, 340(4): 1209-1216.
[58] Zhang M, Barg R, Yin M, et al. Modulated fatty acid desaturation via over expression of two distinct ω-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants [J]. Plant J, 2005, 44: 361-371.

高等植物脂肪酸去饱和酶及编码基因研究进展

Progress of research on fatty acid desaturase and their coding genes in higher plant

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	龚茂佳, 王娟, 付小勇, 寇卫利, 鲁宁, 王秋华, 赖虹燕. 云南广西蒜头果适生区预测及环境影响因子[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 44-52.
[2]	姚正明, 田旭琴, 蒙慧理, 邓云飞. 爵床科凹苞马蓝在贵州的分布新记录及其补充描述[J]. 南京林业大学学报（自然科学版）, 2021, 45(4): 177-182.
[3]	邓睿, 张梅丽, 周明, 郑宝江. 中国茶藨子属1新记录种[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 231-233.
[4]	岳喜良, 秦健, 洑香香, 尚旭岚, 方升佐. 氮素水平对青钱柳叶片主要次生代谢物含量和抗氧化能力的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(2): 35-42.
[5]	何健,刘慧杰,谢磊. 铁线莲属灌木铁线莲组毛茛科研究进展[J]. 南京林业大学学报（自然科学版）, 2018, 42(01): 156-162.
[6]	秦健,刘洋,方升佐,杨万霞,管玲玲,尚旭岚. 光质和光强对青钱柳生长和抗氧化酶活性的影响[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 13-18.
[7]	曹燕妮,茆慧敏,尚旭岚,方升佐,俞思敏. 冲泡条件对青钱柳茶主要内含物浸出规律的影响[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 19-24.
[8]	刘清亮,李垚1,3,方升佐1,2*. 基于MaxEnt模型的青钱柳潜在适宜栽培区预测[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 25-29.
[9]	卢东升,卢帅,潘中超,李佳佳. 水杉内生真菌生物多样性与生态分布[J]. 南京林业大学学报（自然科学版）, 2013, 37(06): 33-36.
[10]	刘玉香,宋晓琛,江香梅,*. 白楠和闽楠染色体核型分析[J]. 南京林业大学学报（自然科学版）, 2013, 37(05): 157-160.
[11]	臧德奎,解孝满,李文清. 山东植物区系新记录[J]. 南京林业大学学报（自然科学版）, 2013, 37(04): 165-166.
[12]	邱帅,丁信誉,席梦利,施季森. 东方百合SRAP体系优化及引物筛选[J]. 南京林业大学学报（自然科学版）, 2013, 37(03): 6-10.
[13]	王艺,丁贵杰. 外生菌根对马尾松幼苗生长、生理特征和养分的影响[J]. 南京林业大学学报（自然科学版）, 2013, 37(02): 97-102.
[14]	关亚丽1，潘琴1，黄敏仁2. 民族植物学与海南黎药资源开发[J]. 南京林业大学学报（自然科学版）, 2009, 33(04): 145-149.
[15]	方炎明. 陆地植物新系统树之诠释与简评[J]. 南京林业大学学报（自然科学版）, 2009, 33(04): 1-7.