Abstract: Based on the concept and method of ecology and economics, by using the continuous forest inventory permanent sampling plot in 2009 as the basic calculate unit, along with the recent research of forest ecological service by foreign researchers, according to the “sample plot→total” technical route, calculation of forest ecological service of Zhejiang province in 2009 was done. The results showed that the forest of Zhejiang province conserved 13.096 billion tons of water resources, which valued 137.771 billion Yuan; fixed 210.681 8 million tons of soil and conserved 9.385 0 million tons of fertility, which valued 32.022 billion Yuan; the forest absorbed 53.790 1 million tons of CO₂ and released 39.270 1 million tons of O₂, which valued 56.872 billion Yuan; the forest produced 391 300 tons of nutrient which valued 4.701 billion Yuan; the 589 400 tons of sulfur dioxide were absorbed and 118.979 4 million tons of dust were captured by the forest, which valued 18.554 billion Yuan; The forest valued 87.563 billion Yuan for conservation of biodiversity and 18.39 billion Yuan for forest tour. In 2009, the forest of Zhejiang province produces a total benefit of 355.873 billion Yuan. The physical quantities of ecological service function were precisely analyzed and estimated by the sampling theory.