利用ISSR和SRAP分子标记对192份油茶种质材料的遗传多样性进行了分析。其中，以ISSR标记筛选出10条引物，共扩增出118个位点，多态性位点104个，多态性百分率88.14 %，相似系数为0.52～1.00；以SRAP标记挑选了12对引物，共扩增出284个位点，多态性位点244个，多态性百分率85.37 %，相似系数为0.65～0.95。对ISSR、SRAP以及二者的混合数据形成的3个遗传相似矩阵进行相关性研究，得到ISSR和SRAP、ISSR和综合数据以及SRAP和综合数据之间均呈极显著相关，说明这两种分子标记对油茶种质材料的遗传多样性分析具有较高的一致性，且SRAP标记具有更高的可信度。聚类分析发现，以ISSR标记在相似系数为0.68时可划分为6个类群，以SRAP标记在相似系数为0.70时也划分为6个类群,且这些油茶种质材料间的亲缘关系较近。但由聚类分析划分的油茶类群来看，这些油茶种质材料遗传背景又比较复杂，具有丰富的遗传多样性。
Abstract
In this paper, two kinds of molecular markers, ISSR and SRAP were used to assess the genetic diversity in 192 accessions of Camellia oleifera. Ten primers were screened from 100 primers of ISSR, 118 bands were amplified, among which 104 bands were polymorphic bands, the percentage of polymorphic band was 8814 %, the Neis genetic similarity coefficients ranged from 052 to 100. Twelve pairs primers of SRAP were selected out, 284 bands were amplified, of which 244 bands (85.37 %) were polymorphic, and the Neis genetic similarity coefficients ranged from 065 to 095. The correlation of the genetic similarity matrix among the ISSR, SRAP and the combination data were higher. It indicated that the two methods had highly consistent in the genetic diversity analysis of C. oleifera, furthermore the SRAP technique was more reliable. According to the UPGMA method, we made the cluster analysis by using the NTSYS software. The 192 germplasms of C. oleifera could be divided into 6 groups at the similarity coefficient of 068 by ISSR marker; they also could be divided into 6 groups at the similarity coefficient of 070 by SRAP marker. This indicated that the C. oleifera germplasms had close relationship. However, the dendrograms cluster analysis showed that some germplasms probably represent different genetic background and high genetic diversity.
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基金
收稿日期：2011-01-26修回日期：2011-09-08
基金项目：“十一五”国家科技支撑计划（2009BADB1B04）
第一作者：彭方仁，教授，博士。E-mail: frpeng@njfu.edu.cn。
引文格式：彭方仁，吴莺莺，郝明灼,等. 利用ISSR和SRAP标记分析油茶遗传多样性[J]. 南京林业大学学报:自然科学版,2012,36(5):19-25.
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