橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析

doi:10.3969/j.jssn.1000-2006.2012.05.006

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南京林业大学学报（自然科学版） ›› 2012, Vol. 36 ›› Issue (05): 37-41.doi: 10.3969/j.jssn.1000-2006.2012.05.006

橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析

周雪梅^1,2，杨礼富¹，王真辉¹，邹智¹，袁坤¹*

1. 中国热带农业科学院橡胶研究所，农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737；
2. 海南大学农学院，海南海口570100

出版日期:2012-09-30 发布日期:2012-09-30
基金资助:
收稿日期：2011-04-16修回日期：2012-06-19
基金项目：中央级公益性科研院所基本科研业务费专项（YWFZX2008-12，2009-12，2010-9）；海南省重点科技项目（90107）；现代农业产业技术体系建设专项资金（CARS-34-02A）
第一作者：周雪梅，硕士生。*通信作者：袁坤，副研究员。E-mail：yuankun628@126.com
引文格式：周雪梅，杨礼富，王真辉,等. 橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析[J]. 南京林业大学学报:自然科学版,2012,36(5):37-41.

Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis

ZHOU Xuemei^1,2, YANG Lifu¹, WANG Zhenhui¹, ZOU Zhi¹, YUAN Kun¹*

1. Rubber Research Institute, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou 571737， China；
2. College of Agriculture, Hainan University, Haikou 570100， China

Online:2012-09-30 Published:2012-09-30

摘要/Abstract

摘要： 橡胶树死皮是影响天然橡胶产量的重要限制因子。通过双向凝胶电泳技术(2-DE)研究了橡胶死皮树与健康树胶乳C-乳清蛋白表达谱的差异。以软件分析后共获得31个差异表达的蛋白点，经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后，搜索NCBInr数据库，结果有10个蛋白点被成功鉴定，其中6个为未知蛋白，其余4个蛋白分别为chain A,structure of ubiquitinlike protein, Rub1；β1,3glucanase；AIR9 protein和flagellar inner dynein arm heavy chain 11。这些蛋白可能在橡胶树死皮发生过程中扮演重要角色。

Abstract: The tapping panel dryness (TPD) in Hevea brasiliensis is a significant limit factor influencing natural rubber production. In this experiment, the twodimensional gel electrophoresis (2DE) was used to study the differentiation of protein expression profiles of latex Cserum between TPD trees and healthy ones of H. brasiliensis. Thirtyone differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) followed by searching for NCBInr database, ten spots were successfully identified. Six of them were identified as the unknown proteins and the remaining 4 were the chain A, structure of ubiquitinlike protein, Rub1; β1, 3glucanase; AIR9 protein and flagellar inner dynein arm heavy chain 11. These proteins might play important roles in the process of TPD occuring.

中图分类号:

S794.1
Q503

周雪梅,杨礼富,王真辉,等. 橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析[J]. 南京林业大学学报（自然科学版）, 2012, 36(05): 37-41.

ZHOU Xuemei, YANG Lifu, WANG Zhenhui, ZOU Zhi, YUAN Kun*. Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis[J].Journal of Nanjing Forestry University (Natural Science Edition), 2012, 36(05): 37-41.DOI: 10.3969/j.jssn.1000-2006.2012.05.006.

参考文献

[1]刘志昕，郑学勤.橡胶树死皮病的发生机理和假说[J].生命科学研究,2002,6(1):83-85.
[2]覃宝祥,胡新文,邓晓东,等.橡胶树死皮病的形成及其机制[J].植物生理学通讯,2005,41(6):827-830.
[3]Suvachittanont W, Wititsuwannakul R. 3Hydroxy3methylglutarylcoenzyme a synthase in Hevea brasiliensis [J]. Phytochemistry,1995,40（3）: 757-761.
[4]Posch A, Chen Z, Wheele C, et al. Characterization and identification of latex allergens by twodimensional electrophoresis and protein microsequencing [J]. J Allergy Clin Immunol, 1997,99（3）: 385-395.
[5]Lacrotte R, Vichitcholchai N, Chrestin H, et al. Protein markers linked to the Tapping Panel Dryness (TPD) of Hevea brasiliensis[C]//. IRRDB Workshop on Tapping Panel Dryness in Hevea brasiliensis. Haikou: CATAS, 1997.
[6]Sookmark U, PujodeRenaud V, Chrestin H, et a1.Characterization of polypeptides accumulated in the latex cytosol of rubber tree affected by the tapping panel dryness syndrome[J]．Plant Cell Physiol，2002，43：1323-1333．
[7]Blum H, Beier H, Gross H. Improved silver staining of plant proteins,RNA and DNA in polyacrylamide gels [J]. Electrophoresis, 1987, 17 (8): 93-99.
[8]Fernandez J, Gharahdaqhi F, Mische S M. Routine identification of proteins from sodium dodecyl sulfatepolyacrylamide gel electrophoresis （SDSPAGE）gel or polyvinyl difluoride membranes using matrix assisted laser desorption/ionizationtime of flightmass spectrometry （MALDITOFMS） [J]. Electrophoresis, 1998, 19（6）: 1036-1045.
[9]Gharahdaghi F,Weinberg C R,Meagher D A, et al. Mass spectrometric identification of proteins from silverstained polyacrylamide gel：a method for the removal of silverions to enhance sensitivity[J]. Electrophoresis, 1999,20 (3)：601-605.
[10]袁坤，王真辉，丁璇，等.巴西橡胶树树皮蛋白质组学分析体系的构建[J].南京林业大学学报：自然科学版，2009, 33(6): 146-149.
[11]Vierstra R D，Callis J. Polypeptide tags, ubiquitous modifiers for plant protein regulation [J]. Plant Mol Biol, 1999, 41: 435-442.
[12]Melchior F. SUMOnonclassical ubiquitin [J]. Annu Rev Cell Dev Biol, 2000, 16: 591-626.
[13]Kurepa J, Walker J M, Smalle J，et al. The small ubiquitinlike modifier (SUMO) protein modification system in Arabidopsis. Accumulation of SUMO1 and 2 conjugates is increased by stress [J]. Biol Chem, 2003, 278: 6862-6872.
[14]Li D, Deng Z, Chen C, et al. Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization [J]. BMC Plant Biol, 2010, 10（1）: 140-152.
[15]Venkatachalam P, Thulaseedharan A, Raghothama K. Identification of expression profiles of tapping panel dryness (TPD) associated genes from the latex of rubber tree (Hevea brasiliensis Muell. Arg.) [J]. Planta, 2007, 226: 499-515.
[16]Anuratha C S, Zen K C, Cole K C, et al. Induction of chitinases and β1, 3glucanases in Rhizoctonia solani infected rice plants: Isolation of an infectionrelated chitinase cDNA clone[J]. Physiol Plant, 1996, 97: 39-46.
[17]Mauch F, Hadwiger L A, Boller T. Antifungal hydrolases in pea tissue: I. Purification and characterization of two chitinases and two β1, 3glucanases differentially regulated during development and in response to fungal infection[J]. Plant Physiol, 1988, 87: 325-333.
[18]Mauch F, MauchMani B, Boller T. Antifungal hydrolases in pea tissue: II. Inhibition of fungal growth by combinations of chitinase and β1, 3 glucanase[J]. Plant Physiol, 1988, 88: 936-942.
[19]SanchezBallesta M T, Zacarias L，Granell A， et al. β1,3Glucanase gene expression as a molecular marker for postharvest physiological disorders in citrus fruit and its hormonal regulation[J]. Plsharvest Bio Tec, 2008, 48(1): 146-149.
[20]Wan L, Zha W, Cheng X, et al. A rice β1, 3glucanase gene Osg1 is required for callose degradation in pollen development [J]. Planta, 2011, 233 (2): 309-323.
[21]闫洁，陈守才，夏志辉. 橡胶树死皮病胶乳C乳清差异表达蛋白质的筛选与鉴定[J].中国生物工程杂志, 2008, 28(6): 28-36.

橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析

Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis

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