<正>用硫酸铵分级沉淀，离子交换纤维素柱层析分离技术，对香菇〔Ｌｅｎｔｉｎｕｌａ（Ｌｅｎｔｉｎｕｓ）ｅ－ｄｏｄｅｓ〕子实体漆酶进行分离纯化，得到单一组分的漆酶，通过ＳＤＳ－ＰＡＧＥ凝胶电泳法，测得其摩尔式量为５５０００。研究表明该酶的最适ｐＨ为２．６，最适温度为７０℃，酶的动力学研究测出米氏常数Ｋｍ值为０．１４２ｍｍｏｌ。

Through systematical process for isolation and purification of laccase from fresh fruit body of Lenlinula edodes using the technique (NH4)2SO4 fractionation and ionexchange chromatography.We obtained one component of laccase,it showed homogeneity in the gel electrophorosis.The molecular weight was 55　000 by SDSPAGE,The optimum pH, temparature were 2.6 ℃ and 70 ℃，respectively. The studies of enzyme kinetics showed Km=0142 mmol