Abstract: Factors influencing rooting and transplanting of tissuecultured plantlets from Pinus densiflora with excellent resistance to pine wilt disease but poor ability of rooting were investigated. The results showed that the induction rate of the adventitious root reached 93% after treating the shoots at 17 ℃ for 5 days, and it was beneficial for adventitious root induction as adding 30—50 mg/L banana juice into the rooting medium with a controlled temperature of 25 ℃. To sterilize the transplant matrix at high temperature or with 0.2% KMnO4 for two times would be helpful for improving the survival ratio of the tissuecultured plantlets. Spraying nutrition solution could stimulate the plantlets growth. This research contributed to improve the root induction and transplanting survival rate, which provided factory style breeding system and practice evidences.