Abstract: Soil respiration was measured using Licor-6400-09 system in the three different urban plantations in Nanjing Forestry University, and its main regulating factors were also measured at the same time during October, 2007 to September, 2008. The three plantation types were Chinese tulip tree, Chinese cedar and mixed plantation of cork oak, deodar cedar and black locust. The results showed that (1) The seasonal fluctuations of soil respiration rates were significant in Chinese tulip tree, Chinese cedar and mixed plantation, and with maximum of 474, 409 and 414 μmol/(m2· s) in summer, respectively, and minimum of 148,1.21 and 085 μmol/(m2· s) in winter, respectively. (2) The annual mean of soil respiration rate was significantly different among three different plantations(p＜0.05). The order of the annual mean of soil respiration rate was: Chinese tulip tree,mixed plantation, and Chinese cedar. (3) Soil respiration was significantly correlated to soil temperature and soil moisture. (4) The Q10 value was different between three plantations, and the Chinese cedar plantation had the largest Q10 value. The results provide theoretical basis for understanding of carbon cycle in urban plantation of this region.