抗松针褐斑病湿地松组培苗生根条件的优化

doi:10.3969/j.jssn.1000-2006.2011.06.012

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南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (06): 59-62.doi: 10.3969/j.jssn.1000-2006.2011.06.012

抗松针褐斑病湿地松组培苗生根条件的优化

安会翠,叶建仁*,吴小芹,刘戈,朱丽华

南京林业大学森林资源与环境学院，江苏省有害生物入侵预防与控制重点实验室，江苏南京210037

出版日期:2011-11-28 发布日期:2011-11-28
基金资助:
收稿日期：2010-12-12修回日期：2011-04-08基金项目：国家林业局“948”项目（2011-4-69）;江苏高校优势学科建设工程资助项目作者简介：安会翠（1984—）,硕士生。*叶建仁（通信作者），教授。Email：jrye@njfu.edu.cn。

Optimization of root formation in microcuttings of diseaserisistant Pinus elliottii

AN Huicui,YE Jianren*,WU Xiaoqin,LIU Ge,ZHU Lihua

Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037,China

Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要： 以抗松针褐斑病湿地松组培苗为研究材料,对影响组培苗不定根发生的条件进行优化。结果表明：在含4.0 mg/L IBA和007 mg/L NAA的WPM培养基中添加0.4 mg/L多效唑对湿地松组培苗不定根的形成和再生植株的成活率有促进作用;湿地松组培苗不定根原基形成后，出瓶移至装有蛭石的穴盘中，有利于不定根的表达，且根茎连接非常好，植株成活率高达85 %；基因型对苗的成活率有决定性的影响，不同家系及同一家系不同无性系间移栽成活率差异较大。

Abstract: The conditions for root formation and development in microcuttings of Pinus elliottii which were resistant to Lecanosticta acicola were optimized. The results showed that the addition of 0.4 mg/L PP333 to WPM medium in combination with 4.0 mg/L IBA and 0.07 mg/L NAA was beneficial to root induction phase. After the formation of adventitious root primordia, the microcuttings were transferred into the disk containing vermiculite, which were favorable to root expression. Using this procedure, the frequency of root induction was up to 100 % and the survival rate of regerated plantlets reached up to 85 %. Significant genotypic variation at both the family and the clonewithinfamily levels was observed in the survival rate of transplanted plantlets.

中图分类号:

S432.2+1

安会翠,叶建仁,吴小芹,等. 抗松针褐斑病湿地松组培苗生根条件的优化[J]. 南京林业大学学报（自然科学版）, 2011, 35(06): 59-62.

AN Huicui,YE Jianren*,WU Xiaoqin,LIU Ge,ZHU Lihua. Optimization of root formation in microcuttings of diseaserisistant Pinus elliottii[J].Journal of Nanjing Forestry University (Natural Science Edition), 2011, 35(06): 59-62.DOI: 10.3969/j.jssn.1000-2006.2011.06.012.

参考文献

[1]朱丽华,程方,秦莉,等.湿地松组培再生植株瓶内菌根化及驯化移栽[J].浙江林学院学报,2010,27(3)398-403.
[2]朱丽华.湿地松、火炬松和黑松的组培繁殖技术研究[D].南京:南京林业大学,2004.
[3]何月秋.湿地松抗病家系组培繁殖技术研究[D].南京:南京林业大学,2003.
[4]Menzies M I,Holden D G,Klomp B K.Recent trends in nursery practice in New Zealand[J].New Forests,2001,22:3-17.
[5]Thorpe T A,Harry I S,Kumar P P.Application of micropropagation to forestry[G]// Debergh P C,Zimmerman R H. Micropropagation.Dordrecht:Kluwer Academic Publishers,1991.〖JP〗
[6]Mohammed G H,Vidaver W E.Root production and plantlet development in tissuecultured conifers[J].Plant Cell,Tissue Organ Cult,1988,14(3):137-160.
[7]朱丽华,张艺,吴小芹.湿地松的组织培养及植株再生[J].南京林业大学学报:自然科学版,2004,28(6):47-51.
[8]Gresshoff P M,Doy C H. Development and differentiation of haploid Lycopersicon esculentum (tomato)[J].Planta,1972,107(2):161-170.
[9]戴培培.湿地松组培快繁体系优化及再生植株性状表现[D].南京:南京林业大学,2008.
[10]戴培培,吴小芹,李玉琴，等.湿地松组培苗驯化移栽技术[J].林业科技开发,2008,22(1):27-29.
[11]曹孜义,刘国民.实用组织培养教程[M].兰州：甘肃科学技术出版社,2003.
[12]Bergmann B A,Stomp A M.Effect of genotype on rooting of hypocotyls and in vitroproduced shoots of Pinus radiata[J].Plant Cell,Tissue and Organ Culture,1994,39（3）：195-202.
[13]朱丽华,吴小芹.湿地松组培苗生根的影响因子[J].东北林业大学学报,2005,33(5):15-18.
[14]何松林,孔德政,杨秋生,等.组织培养容器环境因子调控技术研究进展[J].河南农业大学学报,2003,37(1): 25-32.

抗松针褐斑病湿地松组培苗生根条件的优化

Optimization of root formation in microcuttings of diseaserisistant Pinus elliottii

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