Abstract: The ubiquitous family of 14-3-3(also known as GF14)proteins function as regulators in a variety of stress response processes in plants. Five GF14 genes from Tamarix hispida, designated ThGF14a to ThGF14 e, were identified from an exhaustive search of six cDNA library databases. The basic bioinformatics analysis of five GF14 genes were implemented, and the expression patterns of GF14 genes were analyzed by qRT-PCR under the treatment of stresses. The results were as follows: Bioinformatics analysis showed that ThGF14 proteins contained a highly conserved 14-3-3 domain, and the amino acid sequences are highly conserved except at the N- and C-terminal regions. A phylogenetic analysis indicated that ThGF14 proteins have both ε-like and non-ε forms. The expression profiles of the five ThGF14 genes in response to NaCl, polyethylene glycol(PEG), low temperature, and CdCl₂ for various treatment periods(6, 24, 48, and 72 h)in leaf and root tissues were investigated using qRT-PCR. The results showed that most of the five ThGF14 genes were induced(>2-fold)in leaf and root tissues under abiotic stress, and there were significant differences in response to stress between the various genes. Interestingly, the ThGF14c gene, which generated analogous results under stress in both tissues, was the most highly induced of expression level. Taken together, these results indicate that ThGF14 genes are integral to the abiotic stress response.