青钱柳嫁接愈合过程中砧穗生理特性及其与亲和性的关系

doi:10.12302/j.issn.1000-2006.202010024

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (1): 13-20.doi: 10.12302/j.issn.1000-2006.202010024

所属专题：青钱柳培育与选优专题

• 专题报道(执行主编李维林) • 上一篇下一篇

青钱柳嫁接愈合过程中砧穗生理特性及其与亲和性的关系

李娜¹(), 朱培林², 丰采¹, 温敏学³, 方升佐¹, 尚旭岚¹^,^*()

1.南京林业大学林学院,南方现代林业协同创新中心,江苏南京 210037
2.江西省林业科学院,江西南昌 330032
3.江西省益森实业有限公司,江西宜春 336000

收稿日期:2020-10-17 接受日期:2020-11-15 出版日期:2021-01-30 发布日期:2021-02-01
通讯作者: 尚旭岚
基金资助:
江苏省重点研发计划(现代农业)重点项目(BE2019388);江西省林业局林业科技创新专项([2019]03号)

Variations in physiological characteristics of rootstock-scion and its relationship to graft compatibility during the grafting union process of Cyclocarya paliurus

LI Na¹(), ZHU Peilin², FENG Cai¹, WEN Minxue³, FANG Shengzuo¹, SHANG Xulan¹^,^*()

1. College of Forestry, Co-Innovation center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2. Jiangxi Academy of Forestry, Nanchang 330032, China
3. Jiangxi Yisen Industrial Co.LTD, Yichun 336000, China

Received:2020-10-17 Accepted:2020-11-15 Online:2021-01-30 Published:2021-02-01
Contact: SHANG Xulan

摘要/Abstract

摘要：

【目的】采用不同砧木嫁接青钱柳,分析不同嫁接组合接穗和砧木生理指标的差异,探讨砧穗生理特性与嫁接成活的关系,为青钱柳砧穗嫁接亲和性的早期预测和砧木选择奠定基础。【方法】分别以枫杨(F)、薄壳山核桃(B)和青钱柳(Q)作为砧木嫁接青钱柳接穗(3种嫁接组合),采用切接的方法进行嫁接,测定嫁接后0、40、70 d各嫁接组合砧木和接穗的可溶性糖、可溶性蛋白、淀粉及单宁含量、过氧化物酶(POD)和多酚氧化酶(PPO)活性,并统计嫁接70 d后的成活率。【结果】青钱柳本砧嫁接成活率为53.49%,显著高于以枫杨和薄壳山核桃为砧木的嫁接组合。嫁接后随着时间的延长,本砧嫁接砧穗中的可溶性糖和淀粉含量均呈现先降低后升高的变化规律;3个嫁接组合单宁含量均表现为先升高后降低趋势,但本砧嫁接组合在两个时段均最低,QB组合均最高;3个嫁接组合砧穗POD活性均表现为先升后降;本砧嫁接砧穗PPO活性先降后升,而QF和QB组合砧穗PPO活性均持续升高。相关分析表明,40 d时测定的砧穗可溶性糖、淀粉、单宁含量及PPO活性与嫁接成活率呈显著负相关(P < 0.05),而POD活性与嫁接成活率呈显著正相关(P < 0.05);70 d时测定的砧穗可溶性糖、单宁含量和PPO活性与嫁接成活率呈显著负相关(P < 0.05),但接穗的POD活性与嫁接成活率呈极显著正相关(P < 0.01)。接穗与砧木的PPO和POD活性差值绝对值分别在40和70 d时与嫁接成活率均呈显著负相关(P < 0.05)。【结论】青钱柳本砧嫁接亲和性较强,而以枫杨和薄壳山核桃为砧木亲和性较弱,可溶性糖、淀粉、单宁含量、POD和PPO活性,以及POD和PPO活性差值绝对值可以作为青钱柳嫁接亲和性的早期评价指标。

关键词: 青钱柳, 砧木, 嫁接组合, 成活率, 酶活性

Abstract:

【Objective】 Variations in physiological indexes between scions and rootstocks of different grafting combinations were analyzed in Cyclocarya paliurus, and the relationship between the physiological characteristics of rootstocks and grafting survival was studied. This will allow the early prediction of grafting compatibility of rootstock-scion and the selection of C. paliurus stock. 【Method】 Three grafting combinations were designed, with Pterocarya stenoptera, Carya illinoinensis and C. paliurus as rootstocks to graft C. paliurus (as scions). The contents of soluble sugar, soluble protein, starch and tannin, peroxidase (POD) and polyphenol oxidase (PPO) activity in rootstocks and scions of the grafting combinations were measured at 0, 40 and 70 days after grafting, respectively, while the grafting survival was determined at 70 days after grafting. 【Result】 The graft survival rate with C. paliurus as the rootstock was 53.49%, which was significantly higher than that of the P. stenoptera and C. illinoinensis grafting combinations. With the increase in time after grafting, soluble sugar and starch in the rootstock-scion initially decreased and then rose. The tannin content increased initially and then decreased, and the higher content of tannin in the rootstock-scion was not conducive to the survival of grafting. The increase in POD activity was beneficial for improving the defense response of affinity grafts, while a high PPO activity was not conducive to the survival of grafting. The correlation analysis showed that the soluble sugar, starch, tannin content, and PPO activity of stocks measured at 40 days were significantly negatively correlated with the graft survival rate (P <0.05), while POD activity was significantly positively correlated with the graft survival rate (P <0.05). The soluble sugar, tannin content, and PPO activity of the stocks measured at 70 days were significantly negatively correlated with the graft survival rate (P <0.05), but the POD activity of the scion was significantly positively correlated with the graft survival rate (P <0.01). The absolute value of the PPO activity difference between the scion and rootstock showed a significant negative correlation with the graft survival rate at 40 days (P <0.05), while the absolute value of the POD activity difference at 70 days showed a very significant negative correlation with the graft survival rate (P <0.01). 【Conclusion】 The graft compatibility was strong using C. paliurus as a grafting stock, while the grafting compatibility was weak when P. stenoptera and C. illinoensis were used as rootstocks. The content of soluble sugar, starch, tannin content, POD and PPO activity, and the absolute value of POD and PPO activity can be used as physiological indicators for the early evaluation of the grafting compatibility of rootstock and scion.

Key words: Cyclocarya paliurus, rootstock, grafting combination, survival rate, enzyme activity

中图分类号:

S722

李娜,朱培林,丰采,等. 青钱柳嫁接愈合过程中砧穗生理特性及其与亲和性的关系[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 13-20.

LI Na, ZHU Peilin, FENG Cai, WEN Minxue, FANG Shengzuo, SHANG Xulan. Variations in physiological characteristics of rootstock-scion and its relationship to graft compatibility during the grafting union process of Cyclocarya paliurus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(1): 13-20.DOI: 10.12302/j.issn.1000-2006.202010024.

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参考文献 44

[1]	凌岩, 秦健, 尚旭岚, 等. 施氮量对青钱柳幼苗生长和总酚积累的影响[J]. 植物资源与环境学报, 2020,29(4):45-51.
	LING Y, QIN J, SHANG X L, et al. Effect of applying amount of nitrogen on growth and total phenolic accumulation in Cyclocarya paliurus seedling[J]. J Plant Resour Environ, 2020,29(4):45-51.DOI: 10.3969/j.issn.1674-7895.2020.04.06.
[2]	曹燕妮, 茆慧敏, 尚旭岚, 等. 冲泡条件对青钱柳茶主要内含物浸出规律的影响[J]. 南京林业大学学报(自然科学版), 2017,41(4):19-24.
	CAO Y N, MAO H M, SHANG X L, et al. Effect of brewing conditions on the leaching rate of Cyclocarya paliurus tea compounds[J]. J Nanjing For Univ (Nat Sci Ed), 2017,41(4):19-24. DOI: 10.3969/j.issn.1000-2006.201609044.
[3]	林源, 陈培, 周明明, 等. 天然居群青钱柳叶主要生物活性物质及抗氧化活性研究[J]. 南京林业大学学报(自然科学版), 2020,44(2):10-16.
	LIN Y, CHEN P, ZHOU M M, et al. Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves collected from natural populations[J]. J Nanjing For Univ(Nat Sci Ed), 2020,44(2):10-16. DOI: 10.3969/j.issn.1000-2006.201901045.
[4]	尚旭岚, 李琼琼, 邓波, 等. 光照和施肥对青钱柳幼苗叶片性状与解剖结构的影响[J]. 西南林业大学学报, 2014,34(6):9-15.
	SHANG X L, LI Q Q, DENG B, et al. Effects of light intensity and fertilization on leaf traits and anatomical structure of Cyclocarya paliurus[J]. J Southwest For Univ, 2014,34(6):9-15. DOI: 10.3969/j.issn.2095-1914.2014.06.002.
[5]	CHEN X L, WANG Y, ZHAO H, et al. Localization and dynamic change of saponins in Cyclocarya paliurus (Batal.) Iljinskaja[J]. PLoS One, 2019,14(10):e0223421. DOI: 10.1371/journal.pone.0223421. doi: 10.1371/journal.pone.0223421 pmid: 31613931
[6]	ZHOU M M, LIN Y, FANG S Z, et al. Phytochemical content and antioxidant activity in aqueous extracts of Cyclocarya paliurus leaves collected from different populations[J]. PeerJ, 2019,7:e6492. DOI: 10.7717/peerj.6492. doi: 10.7717/peerj.6492 pmid: 30809459
[7]	张金凤, 方升佐, 尚旭岚, 等. 青钱柳幼胚愈伤组织的诱导[J]. 南京林业大学学报(自然科学版), 2012,36(5):47-50.
	ZHANG J F, FANG S Z, SHANG X L, et al. Callus induction from young embryos of Cyclocarya paliurus[J]. J Nanjing For Univ (Nat Sci Ed), 2012,36(5):47-50. DOI: 10.3969/j.issn.1000-2006.2012.05.008.
[8]	MELNYK C W, MEYEROWITZ E M. Plant grafting[J]. Curr Biol, 2015,25(5):183-188. DOI: 10.1016/j.cub.2015.01.029.
[9]	马绍英, 刘元卫, 苏李维, 等. 葡萄离体嫁接部位的生理生化变化[J]. 果树学报, 2016,33(2):179-186.
	MA S Y, LIU Y W, SU L W, et al. Physiological and biochemical changes in the graft site after in vitro grafting in grape[J]. J Fruit Sci, 2016,33(2):179-186. DOI: 10.13925/j.cnki.gsxb.20150278.
[10]	冯金玲, 杨志坚, 陈辉, 等. 油茶芽苗砧嫁接体的亲和性生理[J]. 福建农林大学学报(自然科学版), 2011,40(1):24-30.
	FENG J L, YANG Z J, CHEN H, et al. Physiological affinity of nurse seed grafted union of Camellia oleifera[J]. J Fujian Agric For Univ (Nat Sci Ed), 2011,40(1):24-30. DOI: 10.13323/j.cnki.j.fafu(nat.sci.).2011.01.014.
[11]	LÓPEZ-GÓMEZ E, SAN JUAN M A, DIAZ-VIVANCOS P, et al. Effect of rootstocks grafting and boron on the antioxidant systems and salinity tolerance of loquat plants (Eriobotrya japonica Lindl.)[J]. Environ Exp Bot, 2007,60(2):151-158. DOI: 10.1016/j.envexpbot.2006.10.007. doi: 10.1016/j.envexpbot.2006.10.007
[12]	严毅. 葡萄柚砧穗愈合过程中酶类活性研究[D]. 昆明:西南林业大学, 2012.
	YAN Y. The enzymes activity research on the process of grapefruit grafting on stock-scion[D]. Kunming:Southwest Forestry University, 2012.
[13]	石雪晖, 王淑英, 吴艳纯, 等. 葡萄叶片中生理生化物质含量与嫁接亲和力关系的研究[J]. 果树学报, 2001,18(1):24-27.
	SHI X H, WANG S Y, WU Y C, et al. Study on the effects of the content of physiological and biochemical substances in the rootstock leaves to the graft compatibility[J]. J Fruit Sci, 2001,18(1):24-27. DOI: 10.3969/j.issn.1009-9980.2001.01.006.
[14]	张燕, 邓思, 沈峰杰, 等. 青钱柳砧穗及品系嫁接亲和力初探[J]. 南方农业, 2017,11(1):39-40,43.DOI: 10.19415/j.cnki.1673-890x.2017.1.013.
[15]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000.
[16]	张晓, 田纪春. 不同小麦多酚氧化酶活性检测方法的比较[J]. 山东农业大学学报(自然科学版), 2008,39(1):15-18.
	ZHANG X, TIAN J C. The comparison of different assays of wheat polyphenol oxidase activity[J]. J Shandong Agric Univ (Nat Sci Ed), 2008,39(1):15-18. DOI: 10.3969/j.issn.1000-2324.2008.01.004.
[17]	韩卫娟, 李加茹, 梁玉琴, 等. 柿果实和叶片中可溶性单宁含量的年变化[J]. 南京林业大学学报(自然科学版), 2015,39(6):61-66.
	HAN W J, LI J R, LIANG Y Q, et al. Annual variation of soluble tannin in the fruits and leaves of persimmon[J]. J Nanjing For Univ(Nat Sci Ed), 2015,39(6):61-66. DOI: 10.3969/j.issn.1000-2006.2015.06.012.
[18]	GOLDSCHMIDT, ELIEZER E. Plant grafting:new mechanisms, evolutionary implications[J]. Frontiers in Plant Science, 2014,5(3) :727. DOI: 10.3389/fpls.2014.00727.
[19]	MANOS P S, SOLTIS P S, SOLTIS D E, et al. Phylogeny of extant and fossil Juglandaceae inferred from the integration of molecular and morphological data sets[J]. Systematic Biology, 2007,56(3):412-30. DOI: 10.1080/10635150701408523. doi: 10.1080/10635150701408523 pmid: 17558964
[20]	胡昳恒. 基于基因组学与转录组学的胡桃科植物系统进化及群体遗传学研究[D]. 西安: 西北大学, 2018.
	HU Y H. Phylogenetic and population genetics of Juglandaceae baced on genomics and transcriptomics[D]. Xi’an: Northwest University, 2018.
[21]	朱晓慧, 杨途熙, 魏安智, 等. 无刺花椒嫁接愈合过程中相关生理指标的变化[J]. 西北林学院学报, 2015,30(2):134-138.
	ZHU X H, YANG T X, WEI A Z, et al. Changes of relative physiological indices involved in the graft union development of Zanthoxylum bungeanum[J]. J Northwest For Univ, 2015,30(2):134-138. DOI: 10.3969/j.issn.1001-7461.2015.02.23.
[22]	马攀, 龚榜初, 江锡兵, 等. 不同砧木嫁接甜柿苗期生长生理特性及亲和性评价[J]. 林业科学研究, 2015,28(4):518-523.
	MA P, GONG B C, JIANG X B, et al. Evaluation on affinity,growth trait and physiology indicators of persimmon grafted on different stocks[J]. For Res, 2015,28(4):518-523. DOI: 10.3969/j.issn.1001-1498.2015.04.010.
[23]	杨邵, 束庆龙, 姚小华, 等. 油茶不同芽苗砧嫁接组合的亲和性生理[J]. 东北林业大学学报, 2015,43(7):19-22,46.
	YANG S, SHU Q L, YAO X H, et al. Physiological affinity of different nurse seed grafted union of Camellia oleifera[J]. J Northeast For Univ, 2015,43(7):19-22,46. DOI: 10.3969/j.issn.1000-5382.2015.07.005.
[24]	苗丽, 李衍素, 范兴强, 等. 植物嫁接体接口愈合机制的研究进展[J]. 植物生理学报, 2017,53(1):17-28.
	MIAO L, LI Y S, FAN X Q, et al. Research advances on mechanism of interface healing of plant grafting[J]. Plant Physiol Commun, 2017,53(1):17-28.DOI: 10.13592/j.cnki.ppj.2016.0413.
[25]	王瑞, 陈永忠, 王湘南, 等. 油茶芽苗砧嫁接愈合过程中砧穗相关生理指标的研究[J]. 西北农林科技大学学报(自然科学版), 2014,42(1):46-50.
	WANG R, CHEN Y Z, WANG X N, et al. Dynamic changes in physiological indexes of Camellia oleifera nurse seed graft in the grafting and recovering process[J]. J Northwest A&F Univ (Nat Sci Ed), 2014,42(1):46-50. DOI: 10.13207/j.cnki.jnwafu.2014.01.026.
[26]	LEE K M, LIM C S, MUNEER S, et al. Functional vascular connections and light quality effects on tomato grafted unions[J]. Sci Hortic, 2016,201:306-317. DOI: 10.1016/j.scienta.2016.02.013. doi: 10.1016/j.scienta.2016.02.013
[27]	曹建华, 林位夫, 陈俊明. 砧木与接穗嫁接亲合力研究综述[J]. 热带农业科学, 2005,25(4):64-69.
	CAO J H, LIN W F, CHEN J M. Studies of affinity between rootstock and scion[J]. Chin J Trop Agric, 2005,25(4):64-69. DOI: 10.3969/j.issn.1009-2196.2005.04.018.
[28]	孙华丽, 宋健坤, 李鼎立, 等. 梨不同嫁接组合嫁接愈合过程中生理动态变化研究[J]. 北方园艺, 2013(16):25-29.
	SUN H L, SONG J K, LI D L, et al. Study on the physiological dynamic change of different graft combinations in pear during the grafted healing process[J]. North Hortic, 2013(16):25-29.
[29]	魏菊, 董胜君, 刘明国, 等. 山杏良种嫁接愈合过程中生理生化特性研究[J]. 沈阳农业大学学报, 2017,48(3):304-310.
	WEI J, DONG S J, LIU M G, et al. Physiological and biochemical characteristics in grafted healing process of Armeniaca sibirica seed[J]. J Shenyang Agric Univ (Soc Ed), 2017,48(3):304-310. DOI: 10.3969/j.issn.1000-1700.2017.03.008.
[30]	张力平, 孙长霞, 李俊清, 等. 植物多酚的研究现状及发展前景[J]. 林业科学, 2005,41(6):157-162. doi: 10.11707/j.1001-7488.20050627
	ZHANG L P, SUN C X, LI J Q, et al. The present conditions and development trend of plant polyphenols research[J]. Sci Silvae Sin, 2005,41(6):157-162. DOI: 10.3321/j.issn:1001-7488.2005.06.027.
[31]	苏文川. 薄壳山核桃嫁接愈合的解剖学和生理生化特性研究[D]. 南京:南京林业大学, 2016.
	SU W C. Research on anatomical, physiological and biochemical traits of union of Carya illinoensis bud grafting[D]. Nanjing:Nanjing Forestry University, 2016.
[32]	USENIK V, KRŠKA B, VIČAN M, et al. Early detection of graft incompatibility in apricot (Prunus armeniaca L.) using phenol analyses[J]. Sci Hortic, 2006,109(4):332-338. DOI: 10.1016/j.scienta.2006.06.011. doi: 10.1016/j.scienta.2006.06.011
[33]	张小红, 赵依杰, 林强, 等. 不同砧木嫁接对甜瓜生理和品质的影响[J]. 农学学报, 2012,2(9):32-35.
	ZHANG X H, ZHAO Y J, LIN Q, et al. Influence of physiological indicators and fruit quality on grafted muskmelon of different stocks[J]. Chin Countrys Well-Off Technol, 2012,2(9):32-35.DOI: 10.3969/j.issn.1007-7774.2012.09.008.
[34]	杨冬冬, 黄丹枫. 西瓜嫁接体发育中木质素合成及代谢相关酶活性的变化[J]. 西北植物学报, 2006,26(2):290-294.
	YANG D D, HUANG D F. Lignin contents and the activities of enzymes related to lignin biosynjournal in the stock and scions of watermelon after grafting[J]. Acta Bot Boreali-Occidentalia Sin, 2006,26(2):290-294. DOI: 10.3321/j.issn:1000-4025.2006.02.011.
[35]	白雪. 薄皮甜瓜嫁接体砧穗愈合过程中的亲和生理机制研究[D]. 南宁:广西大学, 2019.
	BAI X. Study on affinity physiological mechanisms during rootstock healing of thin-skinned melon grafting[D]. Nanning:Guangxi University, 2019.
[36]	IRISARRI P, BINCZYCKI P, ERREA P, et al. Oxidative stress associated with rootstock-scion interactions in pear/quince combinations during early stages of graft development[J]. J Plant Physiol, 2015,176:25-35. DOI: 10.1016/j.jplph.2014.10.015. doi: 10.1016/j.jplph.2014.10.015 pmid: 25543953
[37]	GRIMA-PETTENATI J, GOFFNER D. Lignin genetic engineering revisited[J]. Plant Sci, 1999,145(2):51-65. DOI: 10.1016/S0168-9452(99)00051-5. doi: 10.1016/S0168-9452(99)00051-5
[38]	DOUGLAS C J. Phenylpropanoid metabolism and lignin biosynjournal:from weeds to trees[J]. Trends Plant Sci, 1996,1(6):171-178. DOI: 10.1016/1360-1385(96)10019-4. doi: 10.1016/1360-1385(96)10019-4
[39]	杨瑞. 葡萄砧穗组合生理物质变化与嫁接亲和力的关系[J]. 西北农业学报, 2012,21(12):108-111.
	YANG R. Relationship between the physiological and biochemical substances and the graft affinity of grape rootstock-scion combination[J]. Acta Agric Boreali-Occidentalis Sin, 2012,21(12):108-111. DOI: 10.7606/j.issn.1004-1389.2012.12.021.
[40]	姚焕英, 唐静成, 张鞍灵, 等. 核桃属植物化学成分及生物活性研究[J]. 西北植物学报, 2003,23(9):1650-1655.
	YAO H Y, TANG J C, ZHANG A L, et al. Advances in research of the chemistry and bioactivity of Juglans plants[J]. Acta Bot Boreali-Occidentalia Sin, 2003,23(9):1650-1655. DOI: 10.3321/j.issn:1000-4025.2003.09.036.
[41]	苏美玲. 四种砧木对沃柑嫁接亲和性和生长结果比较研究[D]. 南宁:广西大学, 2019.
	SU M L. Comparisons of grafting compatibility and growth fruit of oran on the four rootstock[D]. Nanning:Guangxi University, 2019.
[42]	张蜀秋, 杨世杰, 马龙彪. 嫁接组合形成过程中两种酶活性的动态变化[J]. 北京农业大学学报, 1990(2) :149-152.
	ZHANG S Q, YANG S J, MA L B. The changes of two enzyme activities during the developmental processes of the graft unions[J]. Journal of Beijing Agricultural University, 1990(2) :149-152.
[43]	史俊燕, 樊金拴, 严江. 酚类物质及其相关酶对核桃嫁接成活的影响[J]. 西北林学院学报, 2005,20(1):80-83.
	SHI J Y, FAN J S, YAN J. The effect of polyphenol and relative enzyme on walnut grafting[J]. J Northwest For Univ, 2005,20(1):80-83. DOI: 10.3969/j.issn.1001-7461.2005.01.021.
[44]	黄曼娜, 孙华丽, 宋健坤, 等. ‘杏叶梨/杜梨’嫁接愈合过程的解剖学与生理学研究[J]. 青岛农业大学学报(自然科学版), 2014,31(3) :177-182.
	HUANG M N, SUN H L, SONG J K, et al. Study on anatomy and physiology of graft healing process in ‘P. armeniaeaefolia/P. betulaefolia’[J]. J Qingdao Agric Univ (Nat Sci Ed), 2014,31(3) :177-182. DOI: 10.3969/J.ISSN.1674-148X.2014.03.005.

类别 item	取样时间/d sampling time		可溶性糖质量分数/% soluble sugar content						可溶性蛋白质量分数/% soluble protein content
类别 item	取样时间/d sampling time		QQ		QF		QB		QQ		QF	QB
接穗 scion	0	1.89±0.08 b		1.79±0.09 b		1.90±0.14 b^*		4.54±0.29 b		4.54±0.24 b		4.54±0.41 b
	40	1.27±0.07 c^*		1.64±0.07 b		1.81±0.06 b^*		5.48±0.27 a^*		5.53±0.32 a		5.44±0.29 a
	70	2.20±0.07 a		2.43±0.10 a		3.42±0.15 a		5.63±0.27 a		5.67±0.18 a		6.05±0.38 a
砧木 rootstock	0	1.89±0.08 b		1.59±0.08 c		1.30±0.08 c		4.54±0.29 c		4.30±0.30 b		4.25±0.23 b
	40	1.59±0.06 c		1.74±0.08 b		2.36±0.14 b		6.46±0.30 a		5.58±0.23 a		6.00±0.41 a
	70	2.14±0.11 a		2.51±0.12 a		3.56±0.14 a		5.65±0.28 b		5.42±0.34 a		5.74±0.20 a

类别 item	取样时间/d sampling time		淀粉质量分数/% starch content						单宁质量分数/% tannin content
类别 item	取样时间/d sampling time		QQ		QF		QB		QQ		QF	QB
接穗 scion	0	1.39±0.07 b		1.39±0.07 c^*		1.39±0.08 b^*		1.60±0.24 b		1.46±0.11 c		1.62±0.13 b
	40	1.05±0.07 c		1.53±0.09 b		1.77±0.08 a^*		2.10±0.20 a		2.68±0.21 a		2.93±0.25 a^*
	70	1.81±0.11 a^*		1.78±0.06 a^*		1.85±0.11 a^*		1.77±0.18 ab		2.24±0.23 b^*		2.72±0.25 a
砧木 rootstock	0	1.39±0.07 b		1.63±0.08 b		2.43±0.10 b		1.65±0.20 b		1.40±0.15 b		1.80±0.09 c
	40	1.03±0.11 c		1.57±0.10 b		2.12±0.17 c		2.26±0.20 a		2.78±0.15 a		3.60±0.22 a
	70	2.52±0.09 a		2.40±0.10 a		3.55±0.05 a		1.97±0.16 ab		2.68±0.14 a		3.05±0.25 b

类别 item	取样时间/d sampling time		POD活性/(U·g^-1) POD activity						PPO活性/(U·g^-1) PPO activity
类别 item	取样时间/d sampling time		QQ		QF		QB		QQ		QF	QB
接穗 scion	0	36.40±3.31 b		36.06±2.83 b^*		36.40±1.75 b^*		10.55±0.65 b		10.55±0.84 c		10.55±0.74 c
	40	112.60 ±5.85 a^*		86.20±3.53 a^*		65.80±0.21 a^*		6.95±0.63 c		19.10±1.64 b		17.20±1.55 b^*
	70	31.00±3.08 b		22.40±1.50 c^*		15.50±1.15 c^*		17.10±175 a		22.25±1.81 a		25.15±1.90 a
砧木 rootstock	0	36.40±3.31 b		101.30±4.17 b		120.80±3.50 b		10.55±0.65 b		10.60±0.87 c		11.50±1.05 b
	40	174.80 ±3.89 a		117.50±3.20 a		140.20±0.60 a		7.85±0.79 c		16.10±1.55 b		21.65±1.17 a
	70	34.30±2.55 b		30.30±2.20 c		35.10±2.26 c		14.10±1.66 a		20.25±1.85 a		24.20±1.77 a

取样时间/d sampling time	因子 factors	可溶性糖含量 soluble sugar content	可溶性蛋白含量 soluble protein content	淀粉含量 starch content	单宁含量 tannin content	POD活性 POD activity	PPO活性 PPO activity
40	接穗	-0.812^**	0.426	-0.900^**	-0.844^**	0.864^**	-0.850^**
	砧木	-0.743^*	0.492	-0.848^**	-0.883^**	0.686^*	-0.900^**
	\|接穗指标-砧木指标\|	-0.052	0.184	-0.620	0.510	-0.381	-0.728^*
70	接穗	-0.724^*	-0.491	-0.142	-0.832^**	0.854^**	-0.846^**
	砧木	-0.789^*	-0.110	-0.561	-0.883^**	0.027	-0.867^**
	\|接穗指标-砧木指标\|	-0.368	-0.544	-0.554	-0.196	-0.818^**	0.069

青钱柳嫁接愈合过程中砧穗生理特性及其与亲和性的关系

Variations in physiological characteristics of rootstock-scion and its relationship to graft compatibility during the grafting union process of Cyclocarya paliurus

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