南京林业大学学报(自然科学版) ›› 1995, Vol. 19 ›› Issue (01): 71-74.doi: 10.3969/j.jssn.1000-2006.1995.01.015

• 研究论文 • 上一篇    下一篇

用自旋标记ESR波谱法测定磷脂的相变温度

封维忠,关世英,包宏   

  1. 南京林业大学基础课部,北京中国林科院
  • 出版日期:1995-03-18 发布日期:1995-02-18

TRANSITION TEMPERATURE MEASUREMENT OF DPPC WITH ESR SPIN LABEL

Feng Weizhong;Guan Shiying;Bao Hong   

  1. Basic Courses Division Nanjing Forestry University Nanjing 210037
  • Online:1995-03-18 Published:1995-02-18

摘要: <正>用自旋标记物Tempo(2,2,6,6-四甲基哌啶-1-氮氧基)标记生物膜磷脂DPPC(二棕榈酰胆碱);用电子顺磁共振波谱仪(ESR)测定该磷脂的相变温度;用差示扫描量热仪(DSC)对结果进行验证和比较,指出自旋标记ESR波谱法测定磷脂相变温度的优点。

Abstract: This paper presents an introduction of transition temperature measurement of DPPC(dipolmitoylphosphatidycholine) labeled with Tempol[2,2,6,6-tetramethylpiperidine-1-oxyl(structurel1].The measurement is done by means of electronic spin resonance spectrometer.At the same time, the differential scanning calorlmeter has been used to check and compare the measured results.The advantage of this measuring method is also discussed in the paper.