Abstract: During 1995～1998, the artificial inoculation test of 40 provenance was carried out to select the resistant masson pine, Pinus massoniana , which were resistant to PWN, Bursaphelenchus xylophilus , in south China. The selection results showed that: The death rate of pathogenesis (DRP) and index of pathogenesis (IP) of three resistant provenance are 0% and 0 respectively. The rate of selection of resistant provenance is 7.5%. The test results also showed that the resistance of 15 year old of 3 selected resistant provenances is stable and reliable.The resistance of masson pine is related to the four main factors that the vigorous growth, the vigorous metabolism, the less content of free amino acid and variant content of second metabolic compounds of GX2, GX3 and GD5 of three provenance were resistant to PWN disease.