南京林业大学学报(自然科学版) ›› 2009, Vol. 33 ›› Issue (02): 5-.doi: 10.3969/j.jssn.1000-2006.2009.02.002
张萍,潘惠新,黄敏仁*,王明庥
出版日期:
2009-04-18
发布日期:
2009-04-18
基金资助:
ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu
Online:
2009-04-18
Published:
2009-04-18
摘要: 采用抑制性消减杂交(suppression subtractive hybridization,SSH)技术,以杨四瘿螨诱导48、72 h的美洲黑杨无性系为实验方(tester),以未经诱导的美洲黑杨无性系为驱动方(driver)构建杨四瘿螨诱导差异表达的抑制消减cDNA文库,共获得了710个克隆,所建cDNA文库的插入片段主要集中在200~1 000 bp之间。通过序列测定拼接和同源性对比分析,获得了美洲黑杨涉及信号传递、能量代谢、胁迫响应、物质运输等功能的差异基因。
中图分类号:
张萍,潘惠新,黄敏仁,等. 杨四瘿螨诱导的美洲黑杨抑制消减文库[J]. 南京林业大学学报(自然科学版), 2009, 33(02): 5-.
ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu. Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)[J].Journal of Nanjing Forestry University (Natural Science Edition), 2009, 33(02): 5-.DOI: 10.3969/j.jssn.1000-2006.2009.02.002.
[1]Diatchenko L , Lauy F C, Campbell A P, et al. Suppression subtractivehy bridization: a method for generating differentially regulated or tissue specific cDNA probes and libraries[J]. Proc Natl Acad Sci USA, 1996, 93(12): 6025-6030. [2]Rebrikov D V, Desai S M, Siebert P D, et al. Suppression subtractive hybridization[J]. Mehtods Mol Biol, 2004, 258: 107-134. [3]Keifer H H. Eriophyid studies B2[M]. State Bur. Entomol: California Dep Agr Special Pub, 1961. [4]Keifer H H. Eriophyid studies B21[M]. State Bur Entomol: California Dep Agr Special Pub, 1966. [5]Morris R C, Filer T H, Solomon J D, et al. Insects and Diseases of Cottonwood. Gen Tech Rep SO8[M]. New Orleans LA: U S Dept of Agriculture, Forest Service, Southern Forest Experiment Station, 1975. [6]Ostry Michael E, Wilson Louis F, McNabb Harold S, et al. A guide to insect, disease, and animal pests of poplars[C]//Agric Handb 677. Washington DC: U S Department of Agriculture, 1988. [7]Coyle D R. Effects of clone, silvicultural, and miticide treatments on cottonwood leafcurl mite (Acari: Eriophyidae) damage in plantation Populus[J]. Environ Entomol, 2002, 31(6): 1000-1008. [8]Coyle D R, Amrine J W. New collection records and host range of the cottonwood leafcurl mite, Tetra loblulifera (Keifer)[J]. Acari Eriophyidae, 2004, 30(1): 3-8. [9]Wrzaczek M, Rozhon W, Jonak C. A Proteasomeregulated glycogen synthase kinase3 modulates disease response in plants[J]. Biol Chem, 2007, 282(8): 5249-5255. [10]Kagaya Y, Hobo T, Murata M, et al.Hattori T1 Abscisic acid induced transcription is mediated by phosphorylation of an abscisic acid response element binding factor , TRAB11[J]. Plant Cell, 2002, 14(12): 3177-3189. [11]Godfrey D, Able A J, Dry I B. Induction of a grapevine germinlike protein (VvGLP3) gene is closely linked to the site of Erysiphe necator infection: a possible role in defense[J]. Mol Plant Microbe Interact, 2007, 20(9): 1112-1125. [12]OrozcoCardenas M, Ryan C A. Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway[J]. Proceedings of the National Academy of Sciences of the USA, 1999, 96: 6553-6557. [13]Guan L M, Scandalios J G. Hydrogen peroxidemediated catalase gene expression in response to wounding[J]. Free Radical Biology and Medicine, 2000, 28: 1182-1190. [14]Lorenzo O, Piqueras R, SanchezSerrano J J, et al.Ethylene response factor1 integrates signals from ethylene and jasmonate pathways in plant defense[J]. Plant Cell, 2003, 15: 165-178. [15]Kahl J, Siemens D H, Aerts R J, et al. Herbivoreinduced ethylene suppresses a direct defense but not a putative indirect defense against an adapted herbivore[J]. Planta, 2000, 210: 336-242. [16]Curto M, Cammafeita E, Lopez J A, et al. Aproteomic approach to study pea(Pisum sativum)responses to powdery mildew (Erysiphe pisi)[J]. Proteomics, 2006(6): 163-174. [17]Anderson J P, Badruzsaufari E, Schenk P M, et al. Antagonistic interaction between abscisic acid and jasmonateethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis[J]. Plant Cell, 2004, 16: 3460-3479. [18]Király L, Hafez YM, Fodor J, et al. Suppression of tobacco mosaic virusinduced hypersensitivetype necrotization in tobacco at high temperature is associated with downregulation of NADPH oxidase and superoxide and stimulation of dehydroascorbate reductase[J]. J Gen Virol, 2008, 89(3): 799-808. [19]Mur L A, Kenton P, Lloyd A J, et al. The hypersensitive response; the centenary is upon us but how much do we know[J]. Exp Bot, 2008, 59(3): 501-20. |
[1] | 许慧慧, 班卓, 王晨雪, 毕泉鑫, 刘肖娟, 王利兵. 文冠果BZR1基因家族鉴定及功能分析[J]. 南京林业大学学报(自然科学版), 2025, 49(2): 12-22. |
[2] | 戚亚, 王改萍, 轩辕欣彤, 彭大庆, 李硕民, 李守科, 曹福亮. 文冠果药用优良无性系评价[J]. 南京林业大学学报(自然科学版), 2025, 49(2): 38-44. |
[3] | 陈升侃, 郭东强, 邓紫宇, 唐庆兰, 廖长琨, 杨植旺, 朱原立, 李昌荣. 斑皮柠檬桉种源树高生长稳定性评价[J]. 南京林业大学学报(自然科学版), 2025, 49(2): 67-74. |
[4] | 姚俊修, 任飞, 王因花, 李庆华, 燕丽萍, 郑岩, 吴德军. 基于荧光SSR标记的接骨木种质资源遗传多样性分析[J]. 南京林业大学学报(自然科学版), 2025, 49(2): 75-82. |
[5] | 柯欣, 费琪, 夏馨蕊, 叶建仁, 朱丽华. 抗松针褐斑病湿地松胚性愈伤组织诱导及体胚产量影响因素的研究[J]. 南京林业大学学报(自然科学版), 2025, 49(1): 87-94. |
[6] | 林强, 徐进, 李上前, 林云斌, 章允清, 欧阳磊. 福建福鼎柳杉种子园半同胞子代遗传变异分析与早期选择[J]. 南京林业大学学报(自然科学版), 2025, 49(1): 78-86. |
[7] | 姜波, 安新民. 基因组精准编辑技术及其在林木育种中的应用[J]. 南京林业大学学报(自然科学版), 2025, 49(1): 11-20. |
[8] | 张伟溪, 丁密, 苏晓华, 李爱平, 王小江, 余金金, 李政宏, 黄秦军, 丁昌俊. 小叶杨×欧洲黑杨杂交F1代生长及叶片解剖结构杂种优势分析与抗旱性评价[J]. 南京林业大学学报(自然科学版), 2025, 49(1): 46-58. |
[9] | 杨袁木, 李娜, 陈新宇, 徐放, 潘文, 张卫华. 红锥种源与无性系的材性变异研究[J]. 南京林业大学学报(自然科学版), 2024, 48(6): 41-50. |
[10] | 闫平玉, 张磊, 王佳兴, 冯可乐, 王浩浩, 张含国. 红松天然种群遗传多样性分析及核心种质构建[J]. 南京林业大学学报(自然科学版), 2024, 48(5): 69-80. |
[11] | 王佳兴, 闫平玉, 孙佰飞, 刘劲宏, 冯可乐, 张含国. 长白落叶松自由授粉家系生长变异及优良家系早期选择[J]. 南京林业大学学报(自然科学版), 2024, 48(5): 81-89. |
[12] | 匡泽宇, 彭冶, 方炎明. 铁冬青花挥发性化合物对中华蜜蜂访花的影响[J]. 南京林业大学学报(自然科学版), 2024, 48(4): 254-260. |
[13] | 刘莉, 瞿印权, 余延浩, 王倩, 洑香香. 青钱柳全基因组SSR位点分析及多态性引物开发[J]. 南京林业大学学报(自然科学版), 2024, 48(4): 67-75. |
[14] | 刘夏岚, 宋子琪, 胡凤荣, 尚旭岚. 青钱柳二倍体和四倍体叶特征比较研究[J]. 南京林业大学学报(自然科学版), 2024, 48(4): 76-84. |
[15] | 马坛, 田野, 王书军, 李文昊, 段启英, 张庆源. 不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应[J]. 南京林业大学学报(自然科学版), 2024, 48(3): 172-180. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||