南京林业大学学报(自然科学版) ›› 2009, Vol. 33 ›› Issue (02): 5-.doi: 10.3969/j.jssn.1000-2006.2009.02.002
张萍,潘惠新,黄敏仁*,王明庥
出版日期:
2009-04-18
发布日期:
2009-04-18
基金资助:
ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu
Online:
2009-04-18
Published:
2009-04-18
摘要: 采用抑制性消减杂交(suppression subtractive hybridization,SSH)技术,以杨四瘿螨诱导48、72 h的美洲黑杨无性系为实验方(tester),以未经诱导的美洲黑杨无性系为驱动方(driver)构建杨四瘿螨诱导差异表达的抑制消减cDNA文库,共获得了710个克隆,所建cDNA文库的插入片段主要集中在200~1 000 bp之间。通过序列测定拼接和同源性对比分析,获得了美洲黑杨涉及信号传递、能量代谢、胁迫响应、物质运输等功能的差异基因。
中图分类号:
张萍,潘惠新,黄敏仁,等. 杨四瘿螨诱导的美洲黑杨抑制消减文库[J]. 南京林业大学学报(自然科学版), 2009, 33(02): 5-.
ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu. Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)[J].Journal of Nanjing Forestry University (Natural Science Edition), 2009, 33(02): 5-.DOI: 10.3969/j.jssn.1000-2006.2009.02.002.
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