南京林业大学学报(自然科学版) ›› 2009, Vol. 33 ›› Issue (04): 8-12.doi: 10.3969/j.jssn.1000-2006.2009.04.002
张萍,潘惠新,黄敏仁*,王明庥
出版日期:
2009-08-18
发布日期:
2009-08-18
基金资助:
ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu
Online:
2009-08-18
Published:
2009-08-18
摘要: 采用抑制性消减杂交(suppression subtractive hybridization,SSH)技术,在构建杨四瘿螨诱导差异表达的抑制消减cDNA文库的基础上,通过RTPCR技术对候选基因进行了验证,其中有6条差异表达候选基因在4个时间点表达量有明显的增强。运用RACE(Rapid Amplification of cDNA Ends)技术克隆获得了一段全长2 053 bp的cDNA克隆,命名为PtWRKY,编码588个氨基酸。
中图分类号:
张萍,潘惠新,黄敏仁,等. 杨树抗杨四瘿螨相关基因的表达分析[J]. 南京林业大学学报(自然科学版), 2009, 33(04): 8-12.
ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu. Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)[J].Journal of Nanjing Forestry University (Natural Science Edition), 2009, 33(04): 8-12.DOI: 10.3969/j.jssn.1000-2006.2009.04.002.
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