质谱技术在蛋白质组学研究中的应用

doi:10.3969/j.jssn.1000-2006.2011.01.024

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南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (01): 103-108.doi: 10.3969/j.jssn.1000-2006.2011.01.024

质谱技术在蛋白质组学研究中的应用

甄艳，施季森

南京林业大学，林木遗传与生物技术省部共建教育部重点实验室，江苏南京210037

出版日期:2011-01-14 发布日期:2011-01-14
基金资助:
收稿日期：2009-12-31修回日期：2010-10-26基金项目：国家自然科学基金项目(31000287);江苏省高校自然科学基础研究项目(10KJB220002)作者简介：甄艳(1976—),副教授,博士。*施季森(通信作者)，教授。Email：jshi@njfu.edu.cn。

Application of mass spectrometry in proteomics studies

ZHEN Yan, SHI Jisen

Application of mass spectrometry in proteomics studiesZHEN Yan, SHI Jisen*（Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China）

Online:2011-01-14 Published:2011-01-14

摘要/Abstract

摘要： 随着蛋白质组学研究的迅速发展，质谱技术已成为应用于蛋白质组学研究中的强有力工具和核心技术。质谱技术的先进性在于为蛋白质组学研究提供的通量和分子信息。笔者重点概述了基于质谱路线的蛋白质组学研究，介绍了基于质谱的定量蛋白质组学﹑翻译后修饰蛋白质组学、定向蛋白质组学、功能蛋白质组学以及基于串联质谱技术的蛋白质组学数据解析的研究进展。

Abstract: With the rapid development of proteomics, mass spectrometry is maturing to be a powerful tool and core technology for proteomics studies during the recent years. The superiority of mass spectrometry lies in providing the throughput and the molecular information, which no other technology can be matched in proteomics. In this review, we made a glance on the outline of mass spectrometrybased proteomics. And then we addressed on the advances of data analysis of mass spectrometrybased proteomics, quantitative mass spectrometrybased proteomics, posttranslational modifications based mass spectrometry, targeted proteomics and functional proteomics basedmass spectrometry.

中图分类号:

甄艳,施季森. 质谱技术在蛋白质组学研究中的应用[J]. 南京林业大学学报（自然科学版）, 2011, 35(01): 103-108.

ZHEN Yan, SHI Jisen. Application of mass spectrometry in proteomics studies[J].Journal of Nanjing Forestry University (Natural Science Edition), 2011, 35(01): 103-108.DOI: 10.3969/j.jssn.1000-2006.2011.01.024.

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质谱技术在蛋白质组学研究中的应用

Application of mass spectrometry in proteomics studies

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