液态混合菌发酵优化纤维素酶组分

doi:10.3969/j.jssn.1000-2006.2011.02.017

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南京林业大学学报（自然科学版） ›› 2011, Vol. 35 ›› Issue (02): 78-82.doi: 10.3969/j.jssn.1000-2006.2011.02.017

液态混合菌发酵优化纤维素酶组分

宋向阳，方浩，欧阳嘉，李鑫，勇强

南京林业大学化学工程学院，林木遗传与生物技术省部共建教育部重点实验室，江苏南京210037

出版日期:2011-04-13 发布日期:2011-04-13
基金资助:
收稿日期：2010-04-29修回日期：2010-12-09 基金项目：国家自然科学基金项目（30871992）；江苏省科技支撑计划（BE2010732）；江苏高校科技创新团队项目作者简介：宋向阳(1965—)，副教授，博士。Email: xiangyangsong@hotmail.com。引文格式：宋向阳，方浩，欧阳嘉,等. 液态混合菌发酵优化纤维素酶组分[J]. 南京林业大学学报:自然科学版,2011,35(2):78-82.

ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger

SONG Xiangyang，FANG Hao，OUYANG Jia，LI Xin，YONG Qiang

Key Laboratory of Forest Genetic and Biotechnology Ministry of Education, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037 China

Online:2011-04-13 Published:2011-04-13

摘要/Abstract

摘要： 在里氏木霉和黑曲霉液态混合条件下培养纤维素酶，分析两个菌种的接种比和延迟黑曲霉的接种时间对产酶的影响，探讨两个菌种发挥协同作用的最佳条件。结果表明：黑曲霉延迟接种48 h及里氏木霉与黑曲霉接种质量比1∶1时，所产纤维素酶的滤纸酶活为1163 μmol/(min·mL)；β-葡萄糖苷酶活为0606 μmol/(min·mL)，β-葡萄糖苷酶活与滤纸酶活比值为0521，比单一里氏木霉产纤维素酶的酶活高，并在后续的酶解效果对比中表现最佳。48 h时的酶解得率为6561 %，高于里氏木霉单一培养时所产纤维素酶的得率5391 %和商品纤维素酶的得率4964 %。说明通过混合发酵，纤维素酶的组分得到了优化。

Abstract: Mixed culture of Trichoderma reesei and Aspergillus niger was investigated to optimize the cellulase components in liquidstate fermentation. A mode of delayed inoculation was applied for cocultivation of T. reesei and A. niger. After cultivation of T. reesei for 48 h, A. niger was inoculated at the 1∶1 inoculum ratio. The βglucosidase activity and filter paper activity of the mixed fungi culture were 0.606 μmol/(min·mL) and 1.163 μmol/(min·mL), respectively, corresponding to a ratio of 0.521, which was higher than that of T. reesei under single cultivation. The yield of enzymatic hydrolysis was 65.61 % by the cocultivated cellulase, which was higher than those of singlecultivated cellulase and commercial cellulase. These results demonstrated that cocultivation of T. reesei and A. niger was suitable for optimization of cellulase components.

中图分类号:

TQ925

宋向阳,方浩,欧阳嘉,等. 液态混合菌发酵优化纤维素酶组分[J]. 南京林业大学学报（自然科学版）, 2011, 35(02): 78-82.

SONG Xiangyang，FANG Hao，OUYANG Jia，LI Xin，YONG Qiang. ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger[J].Journal of Nanjing Forestry University (Natural Science Edition), 2011, 35(02): 78-82.DOI: 10.3969/j.jssn.1000-2006.2011.02.017.

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液态混合菌发酵优化纤维素酶组分

ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger

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