Abstract: The hydrolysis effects and disciplination of steam exploded corn stover catalyzed by cellulose and/or β-glucosidase assisted with exogenous additives were investigated in this paper. Test results showed that Tween-80 was the best exogenous additive for its excellent improvement on the enzymatic hydrolysis yield and enzymes stability. At the Tween-80 loading of 4.0 g/L, the glucose yield and total reduced sugar yield reached 86.85 % and 93.45 % with improved by 7.34 % and 9.59 % compared with the control respectively as steam exploded corn stover was hydrolyzed for 48 h with 15.0 μmol/(min〖DK〗·g) cellualse and 30.0 μmol/(min〖DK〗·g) β-glucosidase, and in this hydrolysate the fermentable glucose accounted to 92.94 % of the total reduced sugar. Under this reaction conditions, 56.83 % of the soluble enzymatic protein, 16.87 % of the β-glucosidase activity and 40.04 % of the cellulase activity could be recovered from the enzymatic hydrolysate of steam exploded corn stover as Tween-80 additives was used.