转TabZIP基因白桦的获得及耐盐性分析

李园园; 杨光; 韦睿; 孙延爽; 郭玉华; 张瑞萍; 刘桂丰

doi:10.3969/j.issn.1000-2006.2013.05.002

转TabZIP基因白桦的获得及耐盐性分析

李园园，杨光，韦睿，孙延爽，郭玉华，张瑞萍，刘桂丰*

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 6-12.

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 6-12. DOI: 10.3969/j.issn.1000-2006.2013.05.002

研究论文

转TabZIP基因白桦的获得及耐盐性分析

李园园¹，杨光¹，韦睿¹，孙延爽¹，郭玉华²，张瑞萍¹，刘桂丰¹*

作者信息 +

TabZIP transferred Betula platyphylla generation and salt tolerance analysis

LI Yuanyuan¹，YANG Guang¹，WEI Rui¹，SUN Yanshuang¹，GUO Yuhua², ZHANG Ruiping¹，LIU Guifeng¹*

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摘要

植物碱性亮氨酸拉链(bZIP)蛋白是一类重要的植物转录因子，该蛋白可与顺式作用元件ABRE等相互作用，使下游许多抗旱、耐盐等抗性相关基因得以表达，从而提高植物的抗逆性。为了获得耐盐白桦转基因新品系，采用农杆菌介导法,将来自于柽柳的转录因子TabZIP基因导入白桦基因组，经qRT-PCR及Western blot检测证明获得了6个转基因株系。在质量分数为 0.4%的NaCl胁迫条件下，比较了转基因株系的相对高生长、盐害指数及叶绿素荧光参数等指标。结果显示：盐胁迫后TB4株系的相对高生长量最大，达到52.4%，较对照(CK)提高了73.5%；且它的盐害指数仅为0.05，盐伤害最轻，而非转基因对照株系为0.19；NaCl胁迫后，转基因株系TB4的叶绿素荧光参数不同于其他株系，它的非光化学淬灭参数(qNP)最大，实际光化学效率(ΦPSⅡ)及光化学淬灭系数(qP)的降幅最小，而最大光量子效率参数(Fv/Fm)略高于胁迫前。这些指标的变化均证实外源TabZIP的表达能够提高TB4转基因株系的耐盐能力。

Abstract

Plant basic leucine zipper (bZIP) proteins was an important group of plant transcription factors, as these proteins could interact with cisacting ABRE elements. These proteins activated the expression of some resistance related genes such as drought and salt tolerant genes. In order to create novel salttolerant transgenic lines, the transcription factor gene TabZIP was imported from Tamarix into the birch genome by using agrobacterium mediated transfection. Six transgenic lines were confirmed by qRTPCR and Western blotting. We compared the relative height growth rate, chlorophyll fluorescence parameters and salt injury index in transgenic lines before and after salt stress treatments. The results showed that the TB4 transgenic line was superior to other transgenic lines after salt stress treatments, with a relatively higher height growth(524%), increased by 73.5% if compared to control. The salt damage of TB4 transgenic gene was the lowest, with a salt injury index of 005, while controls were 019. After salt treatment, TB4 demonstrated different chlorophyll fluorescence parameters from the other transgenic lines, with the highest increase in the nonphotochemical quenching parameter, the lowest decrease in the actual photochemical efficiency of PSⅡ and the photochemical quenching coefficient, and the maximal quantum yield of PSⅡ. The changes in these indicators demonstrated that the expression of exogenous TabZIP could improve the resistance of plants to salt stress, and of these transgenic lines obtained would lay foundation for further research into the resistance mechanism of TabZIP.

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李园园，杨光，韦睿，孙延爽，郭玉华，张瑞萍，刘桂丰*. 转TabZIP基因白桦的获得及耐盐性分析[J]. 南京林业大学学报（自然科学版）. 2013, 37(05): 6-12 https://doi.org/10.3969/j.issn.1000-2006.2013.05.002

LI Yuanyuan，YANG Guang，WEI Rui，SUN Yanshuang，GUO Yuhua, ZHANG Ruiping，LIU Guifeng*. TabZIP transferred Betula platyphylla generation and salt tolerance analysis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(05): 6-12 https://doi.org/10.3969/j.issn.1000-2006.2013.05.002

中图分类号： S71846

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基金

收稿日期：2012-08-24修回日期：2012-11-05 基金项目：国家高技术研究发展计划（2013AA102704）第一作者：李园园，硕士生。 *通信作者：刘桂丰，教授。Email：liuguifeng@126.com。引文格式：李园园，杨光，韦睿，等. 转TabZIP基因白桦的获得及耐盐性分析[J]. 南京林业大学学报：自然科学版，2013， 37（5）：6-12.