利用快相叶绿素荧光参数综合评价3种丁香的耐盐性

张会慧; 张秀丽; 王娟; 田野; 李鑫; 胡举伟; 孙广玉

doi:10.3969/j.issn.1000-2006.2013.05.003

张会慧，张秀丽，王娟，田野，李鑫，胡举伟，孙广玉*

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 13-19.

PDF(1639728 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(1639728 KB)

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 13-19. DOI: 10.3969/j.issn.1000-2006.2013.05.003

研究论文

利用快相叶绿素荧光参数综合评价3种丁香的耐盐性

张会慧，张秀丽，王娟，田野，李鑫，胡举伟，孙广玉*

作者信息 +

A comprehensive evaluation of salt resistance in three clove varieties by the fast fluorescence transient parameters

ZHANG Huihui, ZHANG Xiuli, WANG Juan, TIAN Ye, LI Xin, HU Juwei, SUN Guangyu

Author information +

文章历史 +

摘要

以紫丁香(Syringa oblata)、白丁香(S. oblata var. alba)和暴马丁香(S. reticular var. mandshurica)为试验材料，利用快相叶绿素荧光技术研究了叶片光系统Ⅱ(PSⅡ)对盐胁迫的快速响应，并通过PSⅡ功能参数对丁香耐盐性进行了综合评价。结果表明：盐胁迫下3种丁香叶片的盐害表型特征和耐盐阈值与叶绿素荧光参数的变化趋势基本相符，随着盐浓度的增加，3种丁香叶片PSⅡ电子受体库容量降低，电子由QA向QB的传递过程受到抑制，最终导致PSⅡ向PSⅠ的电子传递能力降低，以吸收光能为基础的光合性能指数受到明显抑制。随着盐浓度的增加，3种丁香叶片的φDo和DIo/RC均呈增加趋势，即在盐胁迫下3种丁香叶片均可以通过增加热耗散的比例来降低激发能的产生，提高叶片的耐盐性，但过高的热耗散比例也降低了电子传递的能量比例(φEo和ETo/RC降低)。3种丁香叶片的各叶绿素荧光参数之间均存在显著或极显著的相关关系，通过隶属函数法对3种丁香叶片PSⅡ功能耐盐能力进行综合评价，结果发现盐胁迫下白丁香的PSⅡ功能耐盐性最强，暴马丁香次之，紫丁香最差。

Abstract

Syringa oblata, S. oblata var. alba and S. reticular var. mandshurica were used as test materials to investigate physiological reactions of photosystem Ⅱ in leaves under NaCl stress using the fast fluorescence transients techniques. And a comprehensive appraisal of photosystem Ⅱ functions on the salttolerance was estimated in clove varieties. The results showed that the salt damage phenotypic characteristics and salt tolerance threshold values were basically the same as the trend of changes in chlorophyll fluorescence parameters in leaves of three clove. Along with the increasing of salt concentration, photosystem Ⅱ functions of three clove varieties were decreased, storage capacity PSⅡ electronic receptors were reduced, and electrons from QA to QB were inhibited. The inhibitions leaded to a reduction on electron transport from PS Ⅱ to PS Ⅰ, and the photosynthetic performance index on energy absorption basis(PIABS) was significantly inhibited. φDo and DIo/RC in leaves of three clove varieties showed an increasing trend. That was to say, the excitation energy could be reduced by increasing the ratio of heat dissipation in leaves of three clove varieties under salt stress, and the leaf salttolerance could be improved. However, the exorbitant ratio of heat dissipation also reduced the energy ratio of electron transport (φEo and ETo/RC were decreased). There were significant or highly significant correlations among different chlorophyll fluorescence parameters in leaves of three clove varieties. The subordinative fuzzy function was used to evaluate comprehensively salt tolerance, results showed that the ordination of salttolerance in photosystem Ⅱ functions from high to low was as follow:S. oblata var. alba > S. reticular var. mandshurica > Syringa oblata.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

张会慧，张秀丽，王娟，田野，李鑫，胡举伟，孙广玉*. 利用快相叶绿素荧光参数综合评价3种丁香的耐盐性[J]. 南京林业大学学报（自然科学版）. 2013, 37(05): 13-19 https://doi.org/10.3969/j.issn.1000-2006.2013.05.003

ZHANG Huihui, ZHANG Xiuli, WANG Juan, TIAN Ye, LI Xin, HU Juwei, SUN Guangyu. A comprehensive evaluation of salt resistance in three clove varieties by the fast fluorescence transient parameters[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(05): 13-19 https://doi.org/10.3969/j.issn.1000-2006.2013.05.003

中图分类号： Q945.1

参考文献

［1］刘贞, 董文宇, 周广柱, 等. 滨海城市盐碱地园林绿化技术探讨[J]. 北方园艺, 2008(4): 180-183. Liu Z, Dong W Y, Zhou G Z, et al. Planting technology probes of saline soil in coastal cities[J]. Northern Horticulture, 2008(4): 180-183.
［2］黄明勇, 杨剑芳, 王怀锋, 等. 天津滨海盐碱土地区城市绿地土壤微生物特性研究[J]. 土壤通报, 2007, 38(6): 1131-1135. Huang M Y, Yang J F, Wang H F, et al. Microbial properties in urban soils in coastal alkalisaline area of Tianjin[J]. Chinese Journal of Soil Science, 2007, 38(6): 1131-1135.
［3］李海梅, 何兴元, 陈玮. 沈阳城市森林主要绿化树种——丁香的光合特性研究[J]. 应用生态学报, 2004, 15(12): 2245- 2249. Li H M, He X Y, Chen W. Photosynthetic characteristics of main urban forest species Syringa oblata in Shenyang[J]. Chinese Journal of Applied Ecology, 2004, 15(12): 2245-2249.
［4］Parida A K, Das A B. Salt tolerance and salinity effects on plants: a review[J]. Ecotoxicology and Environmental Safety, 2005, 60(3): 324-349.
［5］Zhu J K. Salt and drought stress signal transduction in plants[J]. Annual Review of Plant Biology, 2002, 53: 247-273.
［6］Munns R, James R A, Luchli A. Approaches to increasing the salt tolerance of wheat and other cereals[J]. Journal of Experimental Botany, 2006, 57(5): 1025-1043.
［7］张会慧, 张秀丽, 朱文旭, 等. 桑树叶片光系统Ⅱ对NaCl和Na2CO3胁迫的响应[J]. 北京林业大学学报, 2011, 33(6): 15- 20. Zhang H H, Zhang X L, Zhu W X, et al. Responses of photosystem Ⅱ in leaves of mulberry to NaCl and Na2CO3 stress[J]. Journal of Beijing Forestry University, 2011, 33(6): 15-20.
［8］张会慧, 张秀丽, 胡彦波, 等. NaCl和Na2CO3胁迫对桑树幼苗生长及光合特性的影响[J]. 南京林业大学学报: 自然科学版, 2013, 37(1): 217-222. Zhang H H, Zhang X L, Hu Y B, et al. Growth and photosynthetic characteristics in mulberry seedlings under NaCl and Na2CO3 stresses[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2013, 37(1): 217-222.
［9］Zhang J T, Mu C S. Effects of saline and alkaline stresses on the germination, growth, photosynthesis, ionic balance and antioxidant system in an alkalitolerant leguminou forage Lathyrus quinquenervius[J]. Soil Science and Plant Nutrition, 2009, 55(5): 685-697.
［10］张会慧, 张秀丽, 胡彦波, 等. 碱性盐胁迫对桑树幼苗叶片叶绿素荧光特性和激发能分配的影响[J]. 经济林研究, 2012, 30(1): 6-12. Zhang H H, Zhang X L, Hu Y B, et al. Effects of chlorophyll fluorescence characteristics and energy allocation pathways in leaves of mulberry seedlings under alkali salt stress[J]. Nonwood Forest Research, 2012, 30(1): 6-12.
［11］陈锋, 田纪春, 孟庆伟, 等. 短期高温胁迫对高产小麦品系灌浆后期旗叶光系统Ⅱ功能的影响[J]. 应用生态学报, 2006, 17(10): 1854-1858. Chen F, Tian J C, Meng Q W, et al. Effects of shortterm high temperature stress on flag leaf photosystemⅡ functions of highyielding wheat at late grainfilling stage[J]. Chinese Journal of Applied Ecology, 2006, 17(10): 1854-1858.
［12］Ren L L, Ren C M, Zhang W W, et al. Effects of shortterm NaCl stress on photosynthesis in leaves of Glycine soja and Glycine max[J]. Soybean Science, 2009, 28(2): 239-242.
［13］Sharkey T D, Badger M R. Effects of water stress on photosynthetic electron transport, photophosphorylation, and metabolite levels of Xanthium strumarium mesophyll cells[J]. Planta,1982, 156(3): 199 -206.
［14］徐呈祥, 徐锡增. 硅对盐胁迫下金丝小枣叶绿素荧光参数和气体交换的影响[J]. 南京林业大学学报：自然科学版, 2005, 29(1): 25-28. Xu C X, Xu X Z. Effects of silicon on chlorophyll fluorescence and gas exchange of Zizyphus jujuba cv.Jinsi xiaozao under salt stress[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2005, 29(1): 25-28.
［15］王曙光, 孙黛珍, 周福平, 等. 六倍体小黑麦萌发期抗旱性分析[J]. 中国生态农业学报, 2008, 16(6): 1403-1408. Wang S G, Sun D Z, Zhou F P, et al. Analysis of drought resistance of hexaploid triticale at sprouting stage[J]. Chinese Journal of EcoAgriculture, 2008, 16(6): 1403-1408.
［16］王秀玲, 程序, 谢光辉, 等. NaCl胁迫对甜高粱发芽期生理生化特性的影响[J]. 生态环境学报, 2010, 19(10): 2285-2290. Wang X L, Cheng X, Xie G H, et al. Effect of NaCl stress on physiological characteristics of sweet sorghum in sprout stages[J]. Ecology and Environmental Sciences, 2010, 19(10): 2285-2290.
［17］王臣, 虞木奎, 王宗星, 等. 9个楸树无性系对盐胁迫的差异响应[J]. 南京林业大学学报：自然科学版, 2011, 35(2): 20- 24. Wang C, Yu M K, Wang Z X, et al. Difference of response to salt stress of nine clones of Catalpa bungei[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2011, 35(2): 20-24.
［18］张会慧, 张秀丽, 李鑫, 等. 盐胁迫下桑树叶片D1蛋白周转和叶黄素循环对PSⅡ的影响[J]. 林业科学, 2013, 49(1): 99- 106. Zhang H H, Zhang X L, Li X, et al. Role of D1 protein turnover and xanthophylls cycle in protecting of photosystem Ⅱ functions in leaves of Morus alba under NaCl stress[J]. Scientia Silvae Sinicae,, 2013, 49(1): 99-106.
［19］张会慧, 张秀丽, 许楠, 等. 外源钙对干旱胁迫下烤烟幼苗光系统Ⅱ功能的影响[J]. 应用生态学报, 2011, 22(5): 1195- 1200. Zhang H H, Zhang X L, Xu N, et al. Effects of exogenous CaCl2 on the functions of fluecured tobacco seedlings leaf photosystem Ⅱ under drought stress[J]. Chinese Journal of Applied Ecology, 2011, 22(5): 1195-1200.
［20］Jiang C D, Jiang G M, Wang X Z, et al. Increased photosynthetic activities and thermostability of photosystem Ⅱ with leaf development of elm seedlings(Ulmus pumila)probed by the fast fluorescence rise OJIP[J]. Environmental and Experimental Botany, 2006, 58(1): 261-268.
［21］Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria[J]. Photochem Photobiology, 1995, 61(1): 32-42.
［22］孙山, 王少敏, 王家喜, 等. 黑暗中脱水对金太阳杏离体叶片PSⅠ和PSⅡ功能的影响[J]. 园艺学报, 2008, 35(1):1-6. Sun S, Wang S M, Wang J X, et al. Effects of dehydration in the dark on functions of PSⅠ and PSⅡ in apricot (Prunus armeniaca L. ‘Jin Taiyang’) leaves[J]. Acta Horticulturae Sinica, 2008, 35(1):1-6.
［23］李利, 潘响亮, 李宏. 模拟干旱和盐分胁迫对沙枣幼苗PSⅡ活力的影响[J]. 西北植物学报, 2011, 31(4): 768-775. Li L, Pan X L, Li H. Comparative effects of NaCl and polyethylene glycol on PSⅡ activity of Elaeagnus angustifolia [J]. Acta Botanica BorealiOccidentalia Sinica, 2011, 31(4): 768-775.
［24］Davision I R. Stress tolerance in intertidal seaweeds[J]. Journal of Phycology, 1996, 32(2): 197-211.
［25］贾亚雄, 李向林, 袁庆华, 等.披碱草属野生种质资源苗期耐盐性评价及相关生理机制研究[J]. 中国农业科学, 2008, 41 (10): 2999-3007. Jia Y X, Li X L, Yuan Q H, et al. Evaluation of salttolerance and the related physiological characteristics of wild Elymus spp.[J]. Scientia Agricultura Sinica, 2008, 41(10): 2999-3007.

基金

收稿日期：2012-12-13修回日期：2013-06-07 基金项目：“十二五”国家科技支撑计划(2011BAD08B02-3)；国家自然科学基金项目(30771746,31070307)；黑龙江省自然科学基金重点项目(ZD201105) 第一作者：张会慧,博士生。 *通信作者：孙广玉，教授。 Email： sungy@vip.sina.com。引文格式：张会慧，张秀丽，王娟，等. 利用快相叶绿素荧光参数综合评价3种丁香的耐盐性[J]. 南京林业大学学报：自然科学版，2013，37（5）：13-19.