紫丁香叶片发育过程中花色素苷含量与叶绿素荧光和激发能分配的关系

田野; 张会慧; 张秀丽; 王娟; 齐飞; 孙广玉

doi:10.3969/j.issn.1000-2006.2014.01.011

田野,张会慧,张秀丽,王娟,齐飞,孙广玉

南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (01) : 59-64.

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (01) : 59-64. DOI: 10.3969/j.issn.1000-2006.2014.01.011

研究论文

紫丁香叶片发育过程中花色素苷含量与叶绿素荧光和激发能分配的关系

田野, 张会慧, 张秀丽,王娟,齐飞, 孙广玉^*

作者信息 +

The relationship between leaf anthocyanin content and chlorophyll fluorescence, as well as excited energy distribution during leaf expansion of Syringa oblata Lindl.

TIAN Ye, ZHANG Huihui, ZHANG Xiuli, WANG Juan, QI Fei, SUN Guangyu^*

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摘要

以紫丁香(Syringa oblata Lindl.)为试验材料,研究了叶片展开过程中花色素苷含量变化对叶绿素含量、叶绿素荧光特性和光合激发能分配的影响。结果表明:紫丁香叶片从幼叶伸出到叶片成熟的各生育阶段,花色素苷含量降低,而叶绿素含量增加,二者之间呈明显的负相关,叶片对光能的捕获和利用能力也随着花色素苷的降低而增强,但是,从叶片伸出到叶片展开,叶片最大光化学效率(F_v/F_m)没有明显变化。在紫丁香叶片伸出到扩展的进程中,花色素苷含量降低,PSⅡ反应中心吸收的光能分配到失活PSⅡ反应中心的热耗散量子产额(Ф_NF)下降,以及依赖于叶黄素循环的量子产额(Ф_NPQ)下降。说明叶片叶龄越小,花色素苷含量越多,依赖于类囊体膜两侧质子梯度和叶黄素循环耗散的能量比例明显高于成熟叶片,而且PSⅡ反应中心吸收光能分配到无活性反应中心的比例较大,这种光能的分配机制明显降低了幼叶中有活性PSⅡ反应中心的压力,因此,花色素苷可防止幼叶光合机制受到过剩光能的破坏。

Abstract

In this paper, effects of changes in anthayanin content on chlorophyll contents, chlorophyll fluorescence characteristics and excitation energy distribution in photosynthetic apparatus in leaves of Syringa oblata Lindl.during leaf expansion were investigated. The results showed that anthocyanin contents decreased and chlorophyll contents increased in leaves of S. oblata with the expansion of leaves. There was a significant negative relationship between anthocyanin content and chlorophyll content, and capability of light capture and utilization of leaves were strengthened gradually with the increasing of anthocyanin contents, but the maximal PSⅡ photochemical efficiency(F_v/F_m)had no significant change. During leaf expansion, leaf anthocyanin contents decreased, and the quantum yield of thermal dissipation in non-functional PSⅡ(Ф_NF)and the quantum yield of light-dependent and △pH-and xanthophyll-mediated regulated thermal dissipation(Ф_NPQ)decreased. This showed that the younger leaf age was, the more anthocyanin contents had. And not only energy proportion of trans-thylakoid pH gradient and xanthophyll-regulated thermal energy dissipation in young leaves were higher than in mature leaves, but also quantum yield of thermal dissipation assigned by light absorption of PSⅡ reaction center to non-functional PSⅡ was larger. The distribution mechanism of light energy significantly decreased the pressure of active PSⅡ response center in young leaves, therefore, anthocyanin can prevent young leaves form the destruction of excess light energy.

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田野,张会慧,张秀丽,王娟,齐飞,孙广玉. 紫丁香叶片发育过程中花色素苷含量与叶绿素荧光和激发能分配的关系[J]. 南京林业大学学报（自然科学版）. 2014, 38(01): 59-64 https://doi.org/10.3969/j.issn.1000-2006.2014.01.011

TIAN Ye, ZHANG Huihui, ZHANG Xiuli, WANG Juan, QI Fei, SUN Guangyu. The relationship between leaf anthocyanin content and chlorophyll fluorescence, as well as excited energy distribution during leaf expansion of Syringa oblata Lindl.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(01): 59-64 https://doi.org/10.3969/j.issn.1000-2006.2014.01.011

中图分类号： Q945.1

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基金

收稿日期:2012-12-15 修回日期:2013-11-26
基金项目:“十二五”国家科技支撑计划(2011BAD08B02-3); 国家自然科学基金面上项目(31070307,30771746); 黑龙江省自然科学基金重点项目(ZD201105)
第一作者:田野,硕士生。*通信作者:孙广玉,教授。 E-mail:sungy@vip.sina.com。
引文格式:田野, 张会慧, 张秀丽,等. 紫丁香叶片发育过程中花色素苷含量与叶绿素荧光和激发能分配的关系[J]. 南京林业大学学报:自然科学版,2014,38(1):59-64.