PSK对杂交鹅掌楸悬浮培养细胞生长的影响

doi:10.3969/j.issn.1000-2006.2014.01.030

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (01): 171-174.doi: 10.3969/j.issn.1000-2006.2014.01.030

PSK对杂交鹅掌楸悬浮培养细胞生长的影响

张艳娟¹,成铁龙²,周艳威¹,吴霜¹,王鹏凯¹,施季森¹,陈金慧^1*

1. 南京林业大学森林资源与环境学院,林木遗传与生物技术省部共建教育部重点实验室,江苏南京 210037;
2. 中国林业科学研究院科技管理处,北京 100091

出版日期:2014-01-15 发布日期:2014-01-15
基金资助:
收稿日期:2012-07-25 修回日期:2013-07-09
基金项目:国家高技术研究发展计划(2013AA102705); 江苏高校优势学科建设工程资助项目(PAPD); 国家自然科学基金项目(31170619); 教育部新世纪人才基金项目
第一作者:张艳娟,硕士生。*通信作者:陈金慧,教授,博士。E-mail:chenjh@njfu.edu.cn。
引文格式:张艳娟,成铁龙,周艳威,等. PSK对杂交鹅掌楸悬浮培养细胞生长的影响[J]. 南京林业大学学报:自然科学版,2014,38(1):171-174.

Effect of PSK on growth and development of Liriodendron hybrids suspension cultured cells

ZHANG Yanjuan¹, CHENG Tielong², ZHOU Yanwei,¹ WU Shuang¹, WANG Pengkai¹, SHI Jisen¹, CHEN Jinhui^1*

1. Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education,College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China;
2. Division of Research Management, Chinese Academy of Forestry, Beijing 100091, China

Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要： 以杂交鹅掌楸悬浮培养胚性细胞为材料,在培养过程中添加不同浓度的新型植物肽类生长调节物质植物磺肽素(PSK),研究PSK对悬浮细胞生理的影响,分析其在细胞培养中的作用。结果表明:PSK能够促进细胞分裂和增殖,0.8 mg/L的PSK处理后能够使杂交鹅掌楸胚性愈伤组织细胞结构致密、胚性细胞小、直径一致性增加、细胞质变浓,细胞内大量积累淀粉等,同时PSK显著提高了细胞的活力,促进了培养细胞低密度的增殖,并且能够促进蛋白质的积累,有利于杂交鹅掌楸体细胞胚胎的发生。

Abstract: Phytosulfokine(PSK)is a new type of peptide, regulating growth of plants. We studied the role of PSK on suspension culture cells of Liriodendron hybrids. The results showed that PSK promoted cell division and proliferation; under PSK treatment, the embryogenic callus were tight and compacted, and embryogenic cells became smaller,the diameter became more consistent and the cytoplasmic became more rich densely,ect. PSK could also improve cell activity and the accumulation of embryogenic proteins, and could promote proliferation under low-density and could promote somatic embryogenesis of Liriodendron hybrids.

中图分类号:

S792.21

张艳娟,成铁龙,周艳威,等. PSK对杂交鹅掌楸悬浮培养细胞生长的影响[J]. 南京林业大学学报（自然科学版）, 2014, 38(01): 171-174.

ZHANG Yanjuan, CHENG Tielong, ZHOU Yanwei, WU Shuang, WANG Pengkai, SHI Jisen, CHEN Jinhui. Effect of PSK on growth and development of Liriodendron hybrids suspension cultured cells[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(01): 171-174.DOI: 10.3969/j.issn.1000-2006.2014.01.030.

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PSK对杂交鹅掌楸悬浮培养细胞生长的影响

Effect of PSK on growth and development of Liriodendron hybrids suspension cultured cells

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