Abstract: 【Objective】This study was performed in order to provide scientific data for blackberry breeding by a comprehensive evaluation of hybrid strains. 【Methods】 For the five blackberry hybrid strains(5-8-2, 6-6-3, 7-7-4, 7-10-2 and 7-10-6)and their parental cultivars(‘Hull’, ‘Chester’, ‘Triple Crown’, ‘Arapaho’ and ‘Kiowa’), the biological characters(fruit ripening period, thorn trait, SPAD value and growth trait)and economic characters(fruit cluster number, fruit weight, fruit quality and yield)were overall investigated and statistically analyzed, and the comprehensive evaluation value and rank were determined by the analytic hierarchy process(AHP)method. 【Results】Fruit yield and quality were decided as core indicators by comprehensive evaluation for blackberry breeding. The comprehensive evaluation rank of hybrid strain 6-6-3 was class Ⅰ, strain 5-8-2 and cultivar ‘Kiowa’ were class Ⅱ, strains 7-7-4, 7-10-6 and cultivars ‘Hull’, ‘Chester’, ‘Triple Crown’, ‘Arapaho’ were class Ⅲ, strain 7-10-2 was calculated as class Ⅳ. Strain 6-6-3 and 5-8-2 showed outstanding performance of growth and fruiting in Nanjing. 【Conclusion】 Strains 5-8-2 and 6-6-3 are promising candidate of new excellent blackberry cultivar because of their high comprehensive evaluation value and heterobeltiosis, the strains 7-7-4 and 7-10-6 are not clear, strain 7-10-2 should be eliminated in subsequent breeding program due to its low evaluation value.