Abstract: 【Objective】 Investigate the biological effects of ⁶⁰Co-γ radiation on red Freesia refracta(freesia)and purple freesia. 【Method】 In this study, two different varieties of freesia bulbs were irradiated with ⁶⁰Co-γ rays at test doses of 25, 50, 75, and 100 Gy. Statistical analyses of the germination rate, semilethal dose, survival rate, plant height, leaf number, leaf area, root length, flowering rate, pollen viability, and pollen scanning electron microscope after irradiation were conducted. 【Result】 The experimental results demonstrate that the germination rate, plant height, leaf area, root length, flowering rate and pollen vigor of the two freesias treated with five different doses of ⁶⁰Co-γ rays decrease with increasing doses. The optimum dose of freesia(red)is 54.04 Gy, the optimum dose of freesia(purple)is 58.82 Gy, the half flowering dose of freesia(red)is 42.37 Gy, the half flowering dose of freesia(purple)is 39.56 Gy. Pollen surface and pollen morphology gradually increase with increasing irradiation. 【Conclusion】⁶⁰Co-γ rays of various irradiation doses have damage effects on both freesias, and the degree of damage is positively correlated to the radiation dosage. Analyses on pollen can better reflect the plant mutation induced by radiation of the ⁶⁰Co-γ rays. The results can serve as references for the mutation breeding of freesia induced by ⁶⁰Co-γ rays radiation.