南京林业大学学报(自然科学版) ›› 2019, Vol. 62 ›› Issue (01): 76-82.doi: 10.3969/j.issn.1000-2006.201804003
傅仁杰, 祁雪连,丰 凯,夏馨蕊,汤 方*
出版日期:
2019-01-28
发布日期:
2019-01-28
基金资助:
FU Renjie, QI Xuelian, FENG Kai, XIA Xinrui,TANG Fang*
Online:
2019-01-28
Published:
2019-01-28
摘要: 【目的】为了对白蚁进行有效防治,对获得的1株对白蚁具有毒杀作用的菌株进行鉴定与分析。【方法】从感染死亡的黑翅土白蚁上筛选出1株产红色素的菌株。划线分离、纯化后得到较纯的菌株,命名为SM1,将其接种于健康的黑翅土白蚁上,可以使健康的白蚁身体变红死亡。根据菌株SM1形态学特性、生理生化性质、红色色素及16S rDNA的同源性,对其进行了鉴定。【结果】在基础培养基上,菌落呈圆形,易挑起,有轻微臭味,边缘整齐光滑黏稠; SM1直径为1~3 mm,中间朱红色不透明,呈隆起状。菌株SM1经10项生理生化指标测定,确定此菌为粘质沙雷氏菌。此外,克隆得到此菌的16S rDNA片段大小为1 418 bp,与粘质沙雷氏菌的同源性为99%,因此进一步判定该菌为粘质沙雷氏菌。通过超声波辅助丙酮浸提发酵液,可以得到较高纯度的红色色素。将色素溶于乙醇溶液后,用紫外全波长扫描分析。色素在535 nm左右处存在一个明显吸收峰,确定该红色色素是灵菌红素,从而进一步判定该菌为粘质沙雷氏菌。【结论】从黑翅土白蚁上筛选的SM1菌株为粘质沙雷氏菌,研究结果为白蚁的生物防治奠定了理论基础。
中图分类号:
傅仁杰,祁雪连,丰凯,等. 一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性[J]. 南京林业大学学报(自然科学版), 2019, 62(01): 76-82.
FU Renjie, QI Xuelian, FENG Kai, XIA Xinrui,TANG Fang. Identification and characteristics of a strain of Serratia marcescens isolated from the termites,Odontotermes formosanus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 62(01): 76-82.DOI: 10.3969/j.issn.1000-2006.201804003.
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