一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性

doi:10.3969/j.issn.1000-2006.201804003

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南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (01): 76-82.doi: 10.3969/j.issn.1000-2006.201804003

一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性

傅仁杰, 祁雪连,丰凯,夏馨蕊,汤方^*

南京林业大学,南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037

出版日期:2019-01-28 发布日期:2019-01-28
基金资助:
收稿日期:2018-04-02 修回日期:2018-06-08基金项目:江苏省自然科学基金项目(BK20151517); 镇江市重点研发计划-现代农业(NY2017002); 句容市农业科技支撑计划(NY2017846771); 江苏高校优势学科建设工程资助项目( PAPD); 江苏省高等学校大学生创新创业训练计划重点项目(201610298041Z)。第一作者:傅仁杰(596329066@qq.com),负责实验的实施与论文撰写; 祁雪连(1479674671@qq.com),负责实验的操作。*通信作者:汤方(tangfang76@njfu.com.cn),教授,ORCID(0000-0003-3162-6084)。引文格式:傅仁杰, 祁雪连,丰凯,等. 一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性[J]. 南京林业大学学报(自然科学版),2019,43(1):76-82.

Identification and characteristics of a strain of Serratia marcescens isolated from the termites,Odontotermes formosanus

FU Renjie, QI Xuelian, FENG Kai, XIA Xinrui,TANG Fang^*

Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2019-01-28 Published:2019-01-28

摘要/Abstract

摘要： 【目的】为了对白蚁进行有效防治,对获得的1株对白蚁具有毒杀作用的菌株进行鉴定与分析。【方法】从感染死亡的黑翅土白蚁上筛选出1株产红色素的菌株。划线分离、纯化后得到较纯的菌株,命名为SM1,将其接种于健康的黑翅土白蚁上,可以使健康的白蚁身体变红死亡。根据菌株SM1形态学特性、生理生化性质、红色色素及16S rDNA的同源性,对其进行了鉴定。【结果】在基础培养基上,菌落呈圆形,易挑起,有轻微臭味,边缘整齐光滑黏稠; SM1直径为1～3 mm,中间朱红色不透明,呈隆起状。菌株SM1经10项生理生化指标测定,确定此菌为粘质沙雷氏菌。此外,克隆得到此菌的16S rDNA片段大小为1 418 bp,与粘质沙雷氏菌的同源性为99%,因此进一步判定该菌为粘质沙雷氏菌。通过超声波辅助丙酮浸提发酵液,可以得到较高纯度的红色色素。将色素溶于乙醇溶液后,用紫外全波长扫描分析。色素在535 nm左右处存在一个明显吸收峰,确定该红色色素是灵菌红素,从而进一步判定该菌为粘质沙雷氏菌。【结论】从黑翅土白蚁上筛选的SM1菌株为粘质沙雷氏菌,研究结果为白蚁的生物防治奠定了理论基础。

Abstract: 【Objective】 With increasing attention on the ecological environment, biological control of termites needs to be developed.【Method】A strain of a bacteria producing red pigment was screened from Odontotermes formosanus. After being separated and purified, a purer strain was obtained and named SM1. Then, SM1 was inoculated into O. formosanus, which caused the healthy termites to turn red and die. The bacteria was identified based on its morphological ch-aracteristics, physiological and biochemical characteristics, red pigment, and 16S rDNA. 【Result】The bacterial colony was round, easy to pick up, had slight odor, was smooth and sticky, and had a diameter of 1-3 mm and a middle vermilion that was not transparent. In addition, ten physiological and biochemical characteristics of the SM1 strain were measured. These results showed that the bacterium was Serratia marcescens. Furthermore, the size of its cloned 16S rDNA fragment was 1 418 bp, and the homology with Serratia marcescens was 99%. Therefore, the bacteria was determined to be Serratia marcescens. Further, a higher purity of red pigment could be obtained by ultrasonic-assisted acetone extraction, and the pigment was dissolved in ethanol solution and then analyzed by ultraviolet full wavelength scanning. The results showed that the pigment had an obvious absorption peak at 535 nm, which suggested that the red pigment was a prodigiosin. 【Conclusion】The bacterium screened from Odontotermes formosanus was Serratia marcescens, which created a theoretical foundation for the biological control of termites.

中图分类号:

傅仁杰,祁雪连,丰凯,等. 一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性[J]. 南京林业大学学报（自然科学版）, 2019, 43(01): 76-82.

FU Renjie, QI Xuelian, FENG Kai, XIA Xinrui,TANG Fang. Identification and characteristics of a strain of Serratia marcescens isolated from the termites,Odontotermes formosanus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(01): 76-82.DOI: 10.3969/j.issn.1000-2006.201804003.

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一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性

Identification and characteristics of a strain of Serratia marcescens isolated from the termites,Odontotermes formosanus

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